Anti-CD117/c-Kit Rabbit Monoclonal Antibody for WB, FC, ELISA - P05532

Core Product Specifications and Parameters

Biological Background: CD117/c-Kit Function and Localization

• CD117/c-Kit is a single-pass transmembrane tyrosine-protein kinase receptor that specifically binds stem cell factor (KITLG/SCF).
• It plays a master regulatory role in hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, and melanogenesis.
• Upon ligand engagement, KIT activates multiple intracellular cascades, including PI3K/AKT, RAS/MAPK, and JAK/STAT pathways, governing cell survival, proliferation, and differentiation.
• The receptor is primarily localized at the cell membrane, but activated KIT undergoes rapid internalization into the cytoplasm for signal attenuation or degradation.
• Alternative splicing generates several isoforms; isoform 3 is predominantly expressed in hematopoietic stem cells and post-meiotic germ cells, while isoforms 1 and 2 are found in earlier spermatogenic stages and bone marrow.
• Post-translational modifications include ligand-induced phosphorylation, N-linked glycosylation, and ubiquitination, which fine-tune signaling output and receptor turnover.
• Classified as a proto-oncogene, gain-of-function mutations in KIT are implicated in gastrointestinal stromal tumors, mastocytosis, and other malignancies.

Experimental Guidance and Technical Tips

• For Western blotting, expect a major band at approximately 109 kDa corresponding to the full-length receptor; a processed ~22 kDa fragment may also be detected depending on cell type and lysis conditions.
• Because the immunogen spans a large portion of the extracellular domain, this antibody is well-suited for flow cytometry detection of surface CD117 on live, non-permeabilized mouse cells.
• ELISA performance should be optimized with an appropriate capture format; consider pairing with a detection antibody that binds a distinct epitope to avoid competition.
• Validate antibody specificity using a known positive control (e.g., mouse bone marrow cells, mast cell lines) and a negative control where CD117 expression is absent or knocked down.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with a seamless supply chain for IVD raw materials. Our portfolio includes validated anti-CD117/c-Kit antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to support your stem cell and oncology assay development. From rare target sourcing to scalable production, CamelBio connects your project from concept to clinic.