Anti-CD117/c-Kit Polyclonal Antibody for WB and ELISA - P10721

Core Product Specifications and Parameters

Biological Background: CD117/c-Kit Function and Localization

• The KIT gene encodes the mast/stem cell growth factor receptor Kit, also known as CD117, proto-oncogene c-Kit, piebald trait protein (PBT), and SCFR.
• As a receptor tyrosine kinase, KIT binds KITLG/SCF and activates multiple downstream signaling pathways including PI3K-AKT, RAS-MAPK, and JAK-STAT, thereby regulating cell survival, proliferation, and differentiation.
• KIT belongs to the type III receptor tyrosine kinase family and undergoes alternative splicing, generating multiple isoforms with potentially distinct functions.
• The receptor localizes primarily to the cell membrane and can internalize into the cytoplasm upon ligand binding and activation.
• KIT is a glycoprotein with multiple post-translational modifications, including phosphorylation, ubiquitination, and disulfide bond formation, which modulate its activity and stability.
• In testis, KIT is expressed in spermatogonia, Leydig cells, and ejaculated spermatozoa but not in Sertoli cells or spermatocytes. Related references: PMID:20601678
• The protein plays key roles in mast cell development and melanogenesis, and mutations in the KIT gene are associated with piebaldism and various cancers.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 867–976 within the cytoplasmic kinase domain of human CD117/c-Kit, a region highly conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot, expect a band around 110 kDa; consider using appropriate protein extraction buffers and loading controls to confirm specificity.
• In ELISA applications, validate antibody performance using recombinant CD117 protein or cell lysates and optimize the dilution for your experimental system.
• Due to the polyclonal nature, batch-to-batch consistency should be verified, and the antibody may be suitable for other applications after in-house validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for diagnostic manufacturers and research labs, offering one-stop access to high-quality IVD raw materials including this anti-CD117/c-Kit polyclonal antibody for stem cell, oncology, and hematopoiesis studies. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets to accelerate your assay development from concept to clinic.