Anti-CD116/GM-CSFRα Rabbit Monoclonal Antibody for WB, ELISA - P15509

Core Product Specifications and Parameters

Biological Background: CD116/GM-CSFRα Function and Localization

• CD116/GM-CSFRα, encoded by the CSF2RA gene (also known as CSF2R, CSF2RY, CDw116), is the alpha subunit of the receptor for granulocyte-macrophage colony-stimulating factor (GM-CSF).
• It functions as a low-affinity receptor for GM-CSF; ligand binding triggers signal transduction leading to the proliferation, differentiation, and activation of hematopoietic cells.
• The protein is a transmembrane receptor localized to the cell membrane, but alternative splicing generates secreted isoforms.
• As a glycoprotein with disulfide bonds, it undergoes post-translational modifications critical for its structure and function.
• The receptor is implicated in certain disease variants (KEYWORD: Disease variant), highlighting its clinical significance.

Experimental Guidance and Technical Tips

• The immunogen was designed using the extracellular domain (aa 23-320) of human CD116/GM-CSFRα, which may contribute to recognition of membrane-bound and soluble isoforms.
• For Western blot, multiple bands may be observed corresponding to the various alternatively spliced and post-translationally modified forms (protein weight range 27-50 kDa as indicated). Validate in relevant hematopoietic cell lines.
• In ELISA, this antibody could be suitable for capture or detection of the native or recombinant protein. Perform appropriate optimization and include proper controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive one-stop partner for IVD raw materials, providing reliable supply chain solutions for diagnostic manufacturers, labs, and research institutes. This CD116/GM-CSFRα Rabbit mAb supports studies in hematopoiesis and cytokine signaling, and we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to meet your project needs. For custom procurement requirements, contact CamelBio to efficiently bridge the gap from concept to clinic.