Anti-CD115/CSF-1R Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - P07333

Core Product Specifications and Parameters

Biological Background: CD115/CSF-1R Function and Localization

• CD115/CSF-1R encodes the macrophage colony-stimulating factor 1 receptor, a cell-surface tyrosine-protein kinase also known as CSF-1R, M-CSF-R, and proto-oncogene c-Fms (alternative aliases: FMS, CSFR, FIM2, GPSC, HDLS, C-FMS, CD115, HDLS1, BANDDOS).
• Serves as the receptor for both CSF1 and IL34, critically regulating the survival, proliferation, and differentiation of mononuclear phagocytes such as macrophages and monocytes.
• Plays a key role in innate immunity and inflammatory responses by promoting the release of pro-inflammatory chemokines upon ligand engagement.
• Essential for osteoclast proliferation and differentiation, bone resorption, and normal bone/tooth development; also required for male/female fertility and mammary gland morphogenesis during pregnancy.
• Ligand binding triggers multiple signaling cascades—ERK1/2, JNK, AKT1, STAT3/5, SRC kinases—and results in phosphorylation of PIK3R1, PLCG2, GRB2, SLA2, CBL; signaling is down-regulated by phosphatases and receptor internalization. Related references: PMID:20504948, PMID:30982609
• In the central nervous system, may contribute to the development of microglia macrophages. Related references: PMID:30982608
• Localizes to the cell membrane and is predominantly expressed in bone marrow and differentiated blood mononuclear cells; post-translational modifications include glycosylation, disulfide bonds, and ubiquitination, with features typical of immunoglobulin-domain-containing receptors.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the C‑terminal region (aa 873‑972) of human CD115/CSF-1R; this region is likely conserved across variants, but sequence alignment should be performed to predict reactivity with specific isoforms.
• For Western blot, a major band near 108 kDa is anticipated; under reducing and denaturing conditions, confirm the band in positive controls such as macrophage cell lysates (e.g., THP‑1, RAW 264.7) and validate with appropriate blocking and antibody dilution.
• In immunofluorescence and flow cytometry, expect cell‑surface staining; fixation and permeabilization protocols should preserve the epitope. Co‑staining with a membrane marker is recommended to confirm localization.
• The product is cited as cross‑reacting with human, mouse, and rat; however, lot‑to‑lot variability is possible with polyclonal antibodies—re‑validate in your own experimental system and species of interest before proceeding to large‑scale or quantitative assays.
• For ELISA applications, optimize the coating antigen and antibody concentration; the polyclonal nature may improve sensitivity but may also increase background if not titrated properly.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated provider of one-stop IVD raw materials, technical services, and consulting, supporting diagnostic manufacturers from concept to clinic. This anti-CD115/CSF-1R rabbit polyclonal antibody is offered as part of our comprehensive portfolio for macrophage biology, oncology, and immunoassay development. Backed by our extensive sourcing network, we can also supply validated antibody pairs, bulk ancillary reagents, and assist with custom raw-material procurement for rare targets.