Anti-CD112 Rabbit Polyclonal Antibody for WB, ELISA - P32507

Core Product Specifications and Parameters

Biological Background: CD112 (Nectin-2) Function and Localization

• CD112, also known as Nectin-2, herpes virus entry mediator B (HveB), and poliovirus receptor-related protein 2, is a cell adhesion molecule belonging to the nectin family.
• It functions as a modulator of T-cell signaling, acting either as a costimulator or coinhibitor depending on the receptor it binds. Interaction with CD226 stimulates T-cell proliferation and production of cytokines (IL2, IL5, IL10, IL13, IFNG), while interaction with PVRIG inhibits T-cell proliferation.
• CD112 is a probable cell adhesion protein, playing a role in cell-cell interactions.
• The protein localizes to the cell membrane, where it functions as a transmembrane receptor.
• Tissue specificity: expressed in brain, spinal cord, spleen, kidney, heart, and liver.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation; it contains immunoglobulin-like domains characteristic of the nectin family.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 32-345) of mouse CD112, which is well conserved between mouse and human (validated cross-reactivity). This region includes the immunoglobulin-like domains critical for ligand binding.
• For Western blot (WB), consider reducing conditions and validate in relevant mouse tissue lysates (e.g., spleen, brain) or human cell lines; the predicted molecular weight is ~57 kDa (UniProt: 57318 Da).
• In ELISA applications, the polyclonal nature allows flexibility for use as capture or detection reagent; we recommend titrating the antibody individually for each assay system.
• Given the dual role in T-cell signaling, this antibody may be useful in functional studies investigating immune checkpoint modulation (CD226 vs. PVRIG pathways).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop partner for IVD raw-material sourcing, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and access to rare-target raw materials. For T-cell signaling and cell adhesion research, we help you secure key tools with consistent quality, competitive lead times, and technical guidance as needed.