Anti-CD10/MME Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P08473

Core Product Specifications and Parameters

Biological Background: CD10/MME Function and Localization

• CD10/MME encodes Neprilysin (NEP), also called neutral endopeptidase, common acute lymphocytic leukemia antigen (CALLA), and atriopeptidase, a zinc-dependent metalloprotease of the M13 family.
• The protein is anchored to the cell membrane via a single transmembrane helix, with its catalytic domain exposed extracellularly, enabling rapid inactivation of circulating peptide substrates.
• Neprilysin degrades opioid peptides such as Met- and Leu-enkephalins by cleaving the Gly-Phe bond, modulating pain perception and opioid signaling (PubMed:17101991, PubMed:6349683). Related references: PMID:17101991 PMID:6349683
• The enzyme also cleaves vasoactive peptides including bradykinin, substance P, and neurotensin, thereby regulating inflammatory responses and neurotransmission (PubMed:6208535). Related references: PMID:6208535
• It processes angiotensin-1, angiotensin-2, and angiotensin 1-9, influencing blood pressure and electrolyte balance (PubMed:15283675, PubMed:6349683). Related references: PMID:15283675 PMID:6349683
• Neprilysin inactivates atrial natriuretic factor (ANF) and brain natriuretic peptide (BNP), key regulators of cardiovascular homeostasis (PubMed:16254193, PubMed:2531377, PubMed:2972276). Related references: PMID:16254193 PMID:2531377 PMID:2972276
• In skin, CD10 exhibits UV-inducible elastase activity, contributing to the degradation of preelastic and elastic fibers and playing a role in photoaging (PubMed:20876573). Related references: PMID:20876573
• Post-translational modifications include glycosylation, disulfide bond formation, and myristoylation, which are critical for enzyme stability and membrane targeting.
• Mutations in MME are associated with neurological disorders, including Charcot-Marie-Tooth disease type 2T (CMT2T) and spinocerebellar ataxia 43 (SCA43), highlighting its role beyond peptide metabolism.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular catalytic domain (aa 52–352); therefore, the antibody is expected to detect both native and denatured CD10/MME in WB, IHC-P, and ELISA.
• For Western blot, a major band at approximately 86 kDa is anticipated; reducing conditions and adequate blocking are recommended to minimize nonspecific signals.
• In IHC-P, optimize antigen retrieval and use known positive tissues (e.g., kidney, lymphoblastic leukemia) to confirm specificity.
• For ELISA applications, consider using this polyclonal antibody as a capture or detection reagent; pairing with a matched monoclonal antibody may improve sandwich assay performance.
• The reported cross-reactivity with mouse and rat suggests conserved epitopes; validate staining patterns in rodent tissues to ensure specificity before use in translational studies.
• As a membrane protein, enrichment of membrane fractions or use of mild detergents can enhance detection sensitivity in Western blotting.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a comprehensive portfolio of validated antibodies, including this anti-CD10/MME polyclonal antibody, to support assay development for oncology, neurology, and cardiovascular disease markers. With capabilities in bulk antibody supply, custom conjugation, and ancillary reagent provision, we help streamline your IVD raw material procurement from concept to clinic. Let CamelBio be your trusted partner in sourcing rare and critical raw materials for your next-generation diagnostic kits.