Anti-CD10/MME Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P08473

Core Product Specifications and Parameters

Biological Background: Neprilysin (CD10/MME) Function and Localization

• Neprilysin exhibits thermolysin-like specificity restricted to polypeptides up to 30 amino acids, and is biologically important for degrading opioid peptides like Met- and Leu-enkephalins via Gly-Phe bond cleavage. Related references: PMID:15283675, PMID:6208535, PMID:6349683, PMID:17101991
• Cleaves bradykinin, substance P, and neurotensin, modulating inflammatory and pain responses. Related references: PMID:6208535
• Processes angiotensin-1, -2, and 1-9, thereby contributing to the regulation of the renin-angiotensin system and blood pressure. Related references: PMID:15283675, PMID:6349683
• Degrades atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)), key peptides in cardiovascular homeostasis. Related references: PMID:16254193, PMID:2531377, PMID:2972276
• Displays UV-inducible elastase activity toward skin preelastic and elastic fibers, implicating it in skin aging and photoaging. Related references: PMID:20876573
• Located at the cell membrane as a type II integral membrane protein.
• Neprilysin is a glycoprotein, phosphoprotein, and lipoprotein subject to myristoylation. Mutations in MME are linked to Charcot-Marie-Tooth disease type 2T and spinocerebellar ataxia 43.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein spanning amino acids 52-750 of human CD10/MME, encompassing the majority of the extracellular domain; this design may enhance reactivity in native protein detection applications like WB, IHC-P, and IF-P.
• For Western blot, expect a band around 86 kDa. Consider using reducing conditions and validate with positive control lysates from human, mouse, or rat tissues known to express neprilysin.
• As a membrane protein, neprilysin detection via IHC-P or IF-P may require appropriate antigen retrieval and permeabilization techniques; pilot studies are recommended to optimize staining.
• This antibody is reported to work in ELISA, suggesting potential use in quantitative assays; consider pairing with a suitable detection antibody for sandwich ELISA formats.
• While cross-reactivity with mouse and rat is indicated, performance in each species should be independently verified with relevant samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to high-quality IVD raw materials. This anti-CD10/MME antibody supports research into neprilysin's role in cardiovascular, neurological, and inflammatory diseases. Beyond single antibodies, CamelBio offers validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to streamline your assay development and manufacturing processes.