Anti-CD10/MME Monoclonal Antibody for WB, IHC-P, ELISA - P08473

Core Product Specifications and Parameters

Biological Background: Neprilysin Function and Localization

• Neprilysin (CD10/MME) is a type II integral membrane protein belonging to the M13 family of zinc metallopeptidases. It is also known as common acute lymphocytic leukemia antigen (CALLA), enkephalinase, and neutral endopeptidase (NEP).
• It exhibits thermolysin-like specificity, primarily acting on polypeptides of up to 30 amino acids by cleaving peptide bonds on the amino side of hydrophobic residues. Related references: PMID:15283675, PMID:6208535, PMID:6349683
• Neprilysin plays a critical role in the inactivation of opioid peptides Met- and Leu-enkephalins by hydrolyzing the Gly-Phe bond. Related references: PMID:17101991, PMID:6349683
• It also degrades bradykinin, substance P, neurotensin, and various angiotensins (I, II, 1-9), thus modulating vasodilation, pain, and blood pressure. Related references: PMID:6208535, PMID:15283675, PMID:6349683
• Involved in the proteolytic inactivation of atrial natriuretic factor (ANF) and brain natriuretic peptide (BNP), important regulators of cardiovascular and renal homeostasis. Related references: PMID:16254193, PMID:2531377, PMID:2972276
• Displays UV-inducible elastase activity on skin preelastic and elastic fibers, suggesting a role in dermal matrix remodeling. Related references: PMID:20876573
• The protein contains zinc-binding motifs, disulfide bonds, and is post-translationally modified by N-linked glycosylation, myristoylation, and phosphorylation.
• Mutations in the MME gene are associated with Charcot-Marie-Tooth disease type 2T (CMT2T) and spinocerebellar ataxia type 43 (SCA43).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 52–750), making this antibody suitable for detecting native neprilysin on cell surfaces.
• For Western blotting, a band around 86 kDa is expected. Consider using reducing conditions and appropriate loading controls.
• For immunohistochemistry on paraffin-embedded tissues (IHC-P), antigen retrieval steps may enhance detection. Validate staining patterns with known positive and negative controls.
• In ELISA applications, this antibody can serve as a capture or detection reagent when paired with a second CD10/MME antibody recognizing a non-overlapping epitope.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials and technical support. This anti-CD10/MME monoclonal antibody is part of our portfolio for immunoassay development targeting neuropeptide regulation, cardiovascular biomarkers, and oncology research. In addition to validated antibodies, we offer matched antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets.