Anti-CD107b (LAMP2) Rabbit Polyclonal Antibody for WB, ELISA - P17047

Core Product Specifications and Parameters

Biological Background: CD107b/LAMP2 Function and Localization

• LAMP2 is a lysosomal membrane glycoprotein essential for lysosome biogenesis, pH regulation, and autophagy (PMID:10972293; PMID:30718432). Related references: PMID:10972293, PMID:30718432
• It mediates chaperone-mediated autophagy (CMA) by binding target proteins such as GAPDH, GPX4, NLRP3, and MLLT11, targeting them for lysosomal degradation (PMID:30718432). Related references: PMID:30718432
• Required for autophagosome-lysosome fusion, LAMP2 facilitates the accumulation of STX17 on autophagosomes (PMID:27628032). Related references: PMID:27628032
• LAMP2 is critical for normal degradation of autophagosomal contents, particularly under starvation conditions (PMID:10972293; PMID:12221139). Related references: PMID:10972293, PMID:12221139
• Localizes to the membranes of lysosomes, endosomes, autophagosomes, and the plasma membrane.
• Detected in liver and kidney; alternative splicing produces Lamp-2a, Lamp-2b, and Lamp-2c isoforms.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 26–189 of mouse LAMP2, corresponding to the N-terminal luminal domain with multiple N-glycosylation sites. This region may present both linear and conformational epitopes, suitable for detection in denaturing (WB) and native (ELISA) conditions.
• For Western blot, the predicted molecular weight is ~46 kDa, but extensive glycosylation can cause smearing or a larger apparent size. Validate specificity using mouse tissue lysates such as liver or kidney.
• This polyclonal antibody may recognize various LAMP2 splice isoforms (Lamp-2a, Lamp-2b, Lamp-2c). Confirm isoform reactivity in the relevant biological context.
• ELISA applications should be optimized by titrating the antibody and appropriate blocking; cross-reactivity remains limited to mouse according to the supplier's specifications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with researchers and diagnostic developers to streamline sourcing of IVD raw materials for lysosomal biology and autophagy research, including validated antibodies against key targets like CD107b/LAMP2. Our platform offers access to optimized antibody pairs, bulk monoclonal/polyclonal reagents, and rare target sourcing, supported by technical expertise from concept to clinic.