Anti-CD105/Endoglin Monoclonal Antibody for WB, IHC-P, ELISA - P17813

Core Product Specifications and Parameters

Biological Background: Endoglin Function and Localization

• Endoglin is a homodimeric transmembrane glycoprotein and a type I integral membrane protein, featuring a large extracellular domain, a single transmembrane helix, and a short cytoplasmic tail.
• It plays a critical role in the regulation of angiogenesis and is required for normal structure and integrity of the adult vasculature. Related references: PMID:7894484 PMID:21737454 PMID:23300529
• Endoglin acts as a co-receptor for TGF-beta and is involved in both TGFB1/SMAD3 and GDF2/BMP9/SMAD1 signaling pathways, modulating endothelial cell functions. Related references: PMID:21737454 PMID:22347366 PMID:23300529
• It regulates the migration of vascular endothelial cells, contributing to vascular remodeling. Related references: PMID:17540773
• The protein may mediate cell adhesion through binding to integrins and RGD receptors, facilitating endothelial cell attachment. Related references: PMID:1692830
• Endoglin is predominantly expressed on endothelial cells and is detected in placenta and umbilical vein endothelial cells. Related references: PMID:1692830 PMID:10625079
• It undergoes post-translational modifications including phosphorylation, disulfide bond formation, and N-glycosylation, and exists in multiple isoforms due to alternative splicing.
• Mutations in the ENG gene are associated with hereditary hemorrhagic telangiectasia type 1 (HHT1), a vascular dysplasia disorder.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (amino acids 1-200) of human endoglin; therefore, the antibody is expected to recognize the extracellular portion of the protein, suitable for detection of membrane-bound and potentially shed endoglin in WB, IHC-P, and ELISA.
• For WB, note that the predicted molecular weight is approximately 71 kDa, but the mature glycoprotein may run higher due to glycosylation. Optimize denaturing and reducing conditions.
• For IHC-P, antigen retrieval conditions (such as heat-induced epitope retrieval in citrate buffer, pH 6.0) should be validated empirically in the specific tissue and fixation protocol used.
• As this is a recombinant rabbit monoclonal antibody, it offers high specificity and lot-to-lot consistency; consider its use for quantitative ELISA development.
• Cross-reactivity with other species has not been determined; validate in the relevant sample system if non-human samples are used.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to high-quality IVD raw materials, including validated monoclonal antibodies like this anti-CD105/Endoglin antibody for angiogenesis and TGF-beta signaling research. From concept to clinic, we offer comprehensive sourcing support: customized antibody pairs, bulk ancillary reagents, and technical consulting to streamline your diagnostic development. Our portfolio spans rare and common targets, ensuring a reliable supply chain for your critical raw material needs.