Anti-CD105/Endoglin Monoclonal Antibody for ELISA, WB, FC - P17813

Core Product Specifications and Parameters

Biological Background: CD105/Endoglin Function and Localization

• Endoglin (ENG) is a type I membrane glycoprotein and a member of the ZP domain family, expressed as multiple isoforms due to alternative splicing (UniProt: P17813).
• It serves as a TGF-beta coreceptor on endothelial cells, modulating both TGFB1/ALK5/SMAD3 and GDF2/BMP9/ALK1/SMAD1 signaling cascades to regulate angiogenesis and vascular homeostasis. Related references: PMID:21737454, PMID:22347366, PMID:23300529
• Endoglin is essential for normal extraembryonic angiogenesis and embryonic heart development, as well as maintaining adult vascular integrity. Related references: PMID:7894484
• It facilitates endothelial cell migration and may regulate cell shape changes in response to blood flow, driving vascular remodeling. Related references: PMID:17540773
• The protein localizes to the cell membrane and has been detected on endothelial cells and in placenta. Related references: PMID:10625079, PMID:1692830
• Endoglin contains an RGD motif and may mediate cell adhesion by binding to integrins or other RGD receptors. Related references: PMID:1692830
• Post-translational modifications include N-linked glycosylation and disulfide bond formation, with a predicted molecular weight of approximately 70 kDa for the mature glycoprotein.
• Disease-associated variants of ENG are linked to hereditary hemorrhagic telangiectasia type 1 (HHT1), underscoring its clinical significance in vascular disorders.

Experimental Guidance and Technical Tips

• The immunogen used to generate this rabbit monoclonal antibody encompasses the N-terminal extracellular domain (aa 26-337) of human endoglin, which includes the ZP domain; therefore, the antibody may recognize both major splice variants.
• For Western blotting, endoglin typically migrates as a broad band at ~70-80 kDa under reducing conditions due to glycosylation; including deglycosylation controls may help confirm specificity.
• In flow cytometry, the antibody is expected to detect cell surface endoglin on human endothelial cells or endoglin-transfected cell lines; titrate the antibody to optimize staining index.
• When using ELISA, use recombinant human endoglin protein or endoglin-expressing cell lysates as positive controls, and consider validating against known TGF-beta/BMP9-induced biological context.
• Given the role of endoglin in angiogenesis, experimental conditions that modulate TGF-beta signaling pathways (e.g., ligand stimulation, hypoxia) may influence target expression.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in supplying diagnostic manufacturers, laboratories, and research institutes with high-quality IVD raw materials, from validated antibody pairs and bulk ancillary reagents to custom rare-target sourcing. This CD105/Endoglin monoclonal antibody reflects our capability to deliver research-grade immunological tools for angiogenesis and cardiovascular disease applications. By bridging concept-to-clinic needs, we provide a streamlined, reliable sourcing experience to accelerate your assay development and diagnostic pipeline.