Anti-CD105 Rabbit Polyclonal Antibody for WB, ELISA - Q63961

Core Product Specifications and Parameters

Biological Background: Endoglin Function and Localization

• Endoglin (CD105, S-endoglin) is a transmembrane glycoprotein encoded by the Eng gene.
• It plays an important role in the regulation of angiogenesis.
  Related references:
  PMID:10625534
• It regulates the migration of vascular endothelial cells.
  Related references:
  PMID:17540773
• Required for normal extraembryonic angiogenesis and embryonic heart development.
  Related references:
  PMID:10625534
• May regulate endothelial cell shape changes in response to blood flow, driving vascular remodeling.
  Related references:
  PMID:28530658
• Acts as a TGF-beta coreceptor, involved in TGF-beta/BMP signaling and SMAD activation.
  Related references:
  PMID:23300529
• Subcellular localization: Cell membrane.
• Detected on blood vessels, adult pulmonary artery, and endothelial cells in various tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a large extracellular domain (aa 27-581), suggesting the antibody likely recognizes native endoglin; consider testing under non-reducing conditions in WB to preserve disulfide-linked epitopes.
• For Western blot, use appropriate tissue or cell lysates from mouse endothelial cell lines or tissues rich in vasculature; a band near 70 kDa is expected but glycosylation may increase apparent molecular weight.
• In ELISA, immobilize recombinant endoglin or use as a capture/detection antibody after validating performance in your specific assay format.
• Always include positive and negative controls (e.g., endoglin-knockout samples) to confirm specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers streamlined access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-CD105 rabbit polyclonal antibody supports vascular biology and angiogenesis studies, and we can assist with sourcing rare targets and custom reagent development to accelerate your project from concept to clinic. Our comprehensive portfolio and technical support ensure reliable supply for your assay development and manufacturing needs.