Anti-CD103 Rabbit Polyclonal Antibody for WB, ELISA - Q60677

Core Product Specifications and Parameters

Biological Background: CD103 Function and Localization

• CD103, also known as integrin alpha-E or alpha M290, is encoded by the Itgae gene in mouse and belongs to the integrin alpha chain family.
• It forms a heterodimer with integrin beta-7 (ITGB7) to create the alpha-E/beta-7 integrin, which functions as a receptor for E-cadherin.
• This integrin mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers, playing a critical role in mucosal immune surveillance.
• CD103 is a type I transmembrane glycoprotein located on the cell membrane, with a large extracellular domain, a single transmembrane helix, and a short cytoplasmic tail.
• The protein contains typical integrin structural features: calcium-binding sites, disulfide bonds, and multiple FG-GAP repeats in the extracellular region.
• Post-translational modifications include N-linked glycosylation, which contributes to its apparent molecular weight (~129 kDa) in SDS-PAGE, slightly higher than the calculated mass of 128,906 Da.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant fragment (aa 191-385) of mouse CD103; it may recognize multiple epitopes, reducing the risk of signal loss due to minor target variations.
• For Western blot, the predicted molecular weight is ~129 kDa, but glycosylation and other modifications can cause band shifts or smearing; validate band identity using appropriate controls or knockdown samples.
• Mouse tissue lysates from spleen, intestine, or intraepithelial lymphocyte preparations are potential sample sources for CD103 detection.
• In ELISA, consider direct coating of the immunogen or target protein; specificity should be confirmed by testing against irrelevant antigens or null cell lines.
• Cross-reactivity is reported for mouse only; performance with other species should be independently validated.
• Antigen retrieval and blocking steps may need optimization, as integrins can display conformation-dependent epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to IVD raw materials, including validated antibodies against immune-related targets like CD103. Our portfolio covers optimized polyclonal and monoclonal antibodies, matched antibody pairs, and bulk ancillary reagents to support assay development from early R&D to commercial scale. For rare targets or custom sourcing needs, CamelBio helps bridge supply gaps efficiently.