Anti-CD102 Rabbit PolymAb® for WB, IHC-P, ELISA - P13598

Core Product Specifications and Parameters

Biological Background: CD102 (ICAM2) Function and Localization

• CD102, also known as intercellular adhesion molecule 2 (ICAM2), is a member of the immunoglobulin superfamily and a cell adhesion molecule encoded by the ICAM2 gene.
• Functions as a receptor ligand for the integrin LFA-1 (ITGAL:ITGB2) to mediate leukocyte cell-cell adhesion on resting cells. Related references: PMID:10077629 PMID:1676048 PMID:2497351
• Also serves as an endothelial ligand for dendritic cell receptor CD209, triggering transendothelial migration of DC precursors from blood into peripheral tissues and subsequently into lymphoid organs. Related references: PMID:11017109
• Mediates adhesive interactions important for antigen presentation, lymphocyte recirculation, and immune surveillance.
• Constitutively expressed on leukocytes (resting lymphocytes, monocytes, but not neutrophils) and endothelial cells, with abundant expression on vascular endothelial cells and lymphatic vessels in tonsils. Related references: PMID:1676048 PMID:11017109
• Localizes to the cell membrane and microvilli, consistent with its role in mediating cell-cell contact and migration.
• Contains immunoglobulin-like domains, disulfide bonds, and is N-glycosylated, with a predicted molecular weight of approximately 30.7 kDa.

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against recombinant human CD102 protein; epitope may include extracellular Ig domains critical for ligand binding.
• For Western blot (WB): The predicted molecular weight of ICAM2 is ~30 kDa. Consider optimizing reducing conditions to prevent aggregation due to disulfide bonds.
• For IHC-P: Antigen retrieval may be required; validate staining in positive control tissues known to express CD102, such as tonsil or vascular endothelium.
• For ELISA: Consider pairing with a detection antibody that recognizes a non-overlapping epitope to avoid competitive interference.
• Cross-reactivity with mouse and rat ICAM2 suggests potential utility in animal models; however, verify sequence homology and binding affinity in the specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material sourcing, including this anti-CD102 Rabbit PolymAb® for immune adhesion and inflammation research. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline diagnostic development. Our expertise extends to rare target sourcing and technical consultation, supporting your projects from concept to clinic.