Anti-CD101/IGSF2 Rabbit Monoclonal Antibody for ELISA, WB, IF/ICC, FC - Q93033

Core Product Specifications and Parameters

Biological Background: CD101/IGSF2 Function and Localization

• CD101, also known as immunoglobulin superfamily member 2 (IGSF2), cell surface glycoprotein V7, and EWI-101, is a transmembrane protein encoded by the CD101 gene in humans.
• It functions as an inhibitor of T-cell proliferation induced by CD3, suppressing IL2RA expression and IL2 secretion in activated T-cells.
• CD101 inhibits tyrosine kinases required for IL2 production and cellular proliferation, and blocks phospholipase C-gamma-1 (PLCG1) phosphorylation, preventing CD3-induced calcium flux.
• It prevents nuclear translocation of the nuclear factor of activated T-cells, further contributing to its immunosuppressive role.
• CD101 plays a role in T-cell inhibition through IL10 secretion by cutaneous dendritic cells, and is a potential marker of CD4+ CD56+ leukemic tumor cells.
• Subcellularly, CD101 localizes to the plasma membrane as a single-pass type I membrane protein.
• Tissue expression is detected in lung, thymus, small intestine, cutaneous dendritic cells, activated T-cells, monocytes, granulocytes, and certain leukemic cells, including Langerhans cells from Langerhans cell histiocytosis patients.
• It belongs to the immunoglobulin superfamily and contains multiple Ig-like domains, with post-translational modifications including disulfide bonds and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 21–410, corresponding to the extracellular region, making this antibody suitable for detecting membrane-bound CD101 in live-cell FC or IF/ICC, as well as soluble forms in ELISA.
• For WB, the predicted molecular weight is ~115 kDa; consider using a 6–8% SDS-PAGE gel and optimize transfer conditions for high-molecular-weight proteins.
• In FC and IF/ICC, the antibody can be used to detect CD101 on T-cell subsets, dendritic cells, or leukemic blasts; validate in the relevant sample system and consider Fc blocking steps.
• Cross-reactivity with mouse allows translational applications in mouse models; however, verify reactivity and specificity in your model system through positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target procurement to support diagnostic developers from concept to clinic. This anti-CD101/IGSF2 monoclonal antibody is an excellent tool for immunology and oncology research, enabling studies on T-cell regulation and leukemic cell phenotyping. Backed by CamelBio’s comprehensive supply chain, you can source all necessary IVD raw materials and technical services for your assay development needs.