Anti-CD101/IGSF2 Monoclonal Antibody for IF/ICC and Flow Cytometry - Q93033

Core Product Specifications and Parameters

Biological Background: CD101/IGSF2 Function and Localization

• CD101, also known as IGSF2, V7, and EWI-101, is an immunoglobulin superfamily member that functions as a cell surface glycoprotein.
• It acts as an inhibitor of T-cell proliferation induced by CD3, suppressing IL2RA expression and IL2 secretion.
• CD101 inhibits tyrosine kinases required for IL2 production and cellular proliferation, and blocks phospholipase C-gamma-1 phosphorylation and subsequent intracellular calcium flux.
• It prevents nuclear translocation of nuclear factor of activated T-cell (NFAT) to the nucleus, thereby modulating T-cell activation.
• CD101 plays a role in T-cell inhibition via IL10 secretion by cutaneous dendritic cells and may serve as a marker for CD4+ CD56+ leukemic tumor cells.
• Subcellular localization is at the membrane; the protein contains immunoglobulin domains, disulfide bonds, and glycosylation sites.
• Tissue expression includes lung, thymus, and small intestine; it is detected on activated T-cells, monocytes, granulocytes, dendritic cells, and some leukemic cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 21-410), suggesting this antibody targets an extracellular epitope and may be suitable for live-cell staining in flow cytometry.
• For immunofluorescence/ICC, permeabilization may be omitted if only membrane staining is required; however, including permeabilization can help confirm surface localization.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and instrument, and consider using isotype controls.
• Validate the antibody in a relevant cell system, as CD101 expression is upregulated upon T-cell activation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. This anti-CD101/IGSF2 monoclonal antibody addresses T-cell biology and immunology applications, and we can fulfill your broader needs with validated antibody pairs, bulk ancillary reagents, and rare target raw material sourcing. Let our streamlined supply chain accelerate your diagnostic development efficiently.