Anti-CD101 Rabbit Monoclonal Antibody for WB and ELISA - Q93033

Core Product Specifications and Parameters

Biological Background: CD101 Function and Localization

• CD101, also known as immunoglobulin superfamily member 2 (IGSF2), V7, or EWI-101, is a transmembrane glycoprotein belonging to the immunoglobulin superfamily.
• It functions as a negative regulator of T-cell proliferation: it inhibits CD3-induced T-cell activation by blocking IL2RA expression, IL2 secretion, and intracellular calcium mobilization.
• CD101 suppresses phospholipase C-gamma-1 phosphorylation and prevents nuclear translocation of NFAT, thereby limiting IL2 production.
• In cutaneous dendritic cells, CD101 contributes to T-cell inhibition through IL10 secretion.
• Expressed on the plasma membrane, CD101 is found in lung, thymus, small intestine, and on immune cells including activated T-cells, monocytes, granulocytes, and dendritic cells.
• It is a recognized marker for CD4+ CD56+ leukemic tumor cells and is present on Langerhans cells from Langerhans cell histiocytosis patients.
• Contains immunoglobulin-like domains and a signal peptide, with a predicted molecular weight of ~115 kDa.

Experimental Guidance and Technical Tips

• The immunogen (aa 21-410) covers the extracellular region; the antibody may recognize native and denatured CD101. Consider testing under non-reducing and reducing conditions for Western blot.
• For ELISA, validate with recombinant CD101 or lysates from CD101-expressing cells (e.g., activated T-cells, dendritic cells).
• Activation of T-cells (e.g., with CD3/CD28) can upregulate CD101 expression; include appropriate controls and stimulation protocols to enhance detection.
• Reported molecular weight is 115 kDa; verify band migration in your experimental system and use appropriate protein standards.
• Cross-reactivity has been confirmed only for human; if working with other species, perform independent validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. The CD101 rabbit mAb supports immuno-oncology and immune profiling applications; CamelBio can assist in sourcing validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for your assay development needs. We also specialize in rare target raw-material procurement to streamline your research and production workflows.