Anti-CD101 Polyclonal Antibody for WB, ELISA - Q93033

Core Product Specifications and Parameters

Biological Background: CD101 Function and Localization

• CD101, also known as Immunoglobulin superfamily member 2 (IGSF2), cell surface glycoprotein V7, or EWI-101, is a transmembrane glycoprotein containing immunoglobulin domains.
• It functions as a negative regulator of T-cell proliferation induced by CD3, inhibiting IL2 receptor alpha (IL2RA) expression and IL2 secretion.
• CD101 suppresses key signaling events including tyrosine kinase activation, phospholipase C-gamma-1 (PLCG1) phosphorylation, and intracellular calcium mobilization upon CD3 engagement, preventing nuclear translocation of nuclear factor of activated T-cells (NFAT).
• This protein is also involved in T-cell inhibition through IL-10 secretion by cutaneous dendritic cells, suggesting a role in immune tolerance.
• CD101 is expressed in lung, thymus, small intestine, and immune cells such as cutaneous dendritic cells, activated T-cells, monocytes, and granulocytes, as well as in epithelial cells with dendritic morphology.
• It is notably expressed in a subset of leukemic cells, particularly CD4+ CD56+ blastic tumor cells and Langerhans cells from Langerhans cell histiocytosis, serving as a potential disease marker.
• Subcellularly, CD101 localizes to the cell membrane and features structural attributes including disulfide bonds, glycosylation, immunoglobulin domains, and a transmembrane helix (UniProt keywords: Disulfide bond, Glycoprotein, Immunoglobulin domain, Membrane, Transmembrane).

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 21–410 of human CD101, covering most of the extracellular region; this polyclonal antibody may recognize multiple epitopes within this domain.
• For Western blotting, a predominant band at approximately 115 kDa is expected, consistent with the calculated molecular weight of CD101 (UniProt: 115,109 Da); equivalent loading controls and molecular weight markers are recommended.
• In ELISA, the recombinant immunogen can serve as a positive control to optimize coating conditions and to establish assay linearity and sensitivity.
• Since cross-reactivity is verified for human only, this antibody is suited for human samples; reactivity with other species should be assessed experimentally if needed.
• Titrate the antibody empirically for each application and sample type, as polyclonal antibodies may exhibit lot-to-lot variation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for sourcing high-quality IVD raw materials. For CD101 research and diagnostic development, we offer a suite of validated antibodies and recombinant proteins to support your immunoassay and translational projects. From bulk ancillary reagents to technical consultation, our integrated supply chain bridges the gap from concept to clinic.