Anti-CD10 Rabbit Monoclonal Antibody for WB, FC, ELISA - Q61391

Core Product Specifications and Parameters

Biological Background: Neprilysin Function and Localization

• Neprilysin (CD10) is a zinc-dependent metalloprotease belonging to the thermolysin-like family, exhibiting specificity for polypeptides of up to 30 amino acids (By similarity).
• Plays a critical role in the inactivation of opioid peptides such as Met- and Leu-enkephalins by cleaving a Gly-Phe bond (By similarity).
• Catalyzes the hydrolysis of several regulatory peptides, including bradykinin, substance P, and neurotensin (By similarity).
• Cleaves angiotensin-1, angiotensin-2, and angiotensin 1-9, contributing to the regulation of the renin-angiotensin system (By similarity).
• Involved in the degradation of atrial natriuretic factor (ANF), thereby modulating cardiovascular homeostasis (By similarity).
• Exhibits UV-inducible elastase activity toward skin preelastic and elastic fibers, linking it to skin extracellular matrix remodeling (PubMed:20876573). Related references: PMID:20876573
• Localizes primarily to the cell membrane as a type II integral membrane protein (signal-anchor).
• Post-translational modifications include glycosylation, disulfide bond formation, phosphorylation, myristoylation, and anchoring via a transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 52-750 of mouse CD10, representing the large extracellular domain; therefore, the antibody is expected to recognize native cell surface CD10. For flow cytometry, consider using live, non-permeabilized cells to detect surface expression.
• For Western blotting, the predicted protein weight is 86 kDa; however, due to glycosylation, the observed band may appear slightly larger. Validate the band specificity in your target tissue or cell lysate.
• In ELISA applications, use an appropriate recombinant or native CD10 protein as a standard to optimize assay conditions.
• Cross-reactivity is validated for mouse; if using in other species, verify sequence homology and perform pilot experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and research laboratories worldwide. This anti-CD10 rabbit monoclonal antibody supports projects involving peptide metabolism, cardiovascular regulation, and dermatological research. Beyond standard catalog products, CamelBio excels in rare target raw-material sourcing and can tailor supply solutions to accelerate your assay development from concept to clinic.