Anti-CD10 Rabbit Monoclonal Antibody () for Flow Cytometry - P08473

Core Product Specifications and Parameters

Biological Background: CD10 (Neprilysin/CALLA) Function and Localization

• CD10 (Neprilysin) is a cell membrane zinc metalloprotease with thermolysin-like specificity, acting on polypeptides up to 30 amino acids. It cleaves the Gly-Phe bond of enkephalins and degrades various signaling peptides including bradykinin, substance P, neurotensin, and angiotensins. Related references: PMID:15283675 PMID:17101991 PMID:6208535 PMID:6349683
• It plays a crucial role in attenuating natriuretic peptide signaling by degrading atrial natriuretic factor (ANF) and brain natriuretic factor (BNP). Related references: PMID:16254193 PMID:2531377 PMID:2972276
• CD10 exhibits UV-inducible elastase activity, targeting skin preelastic and elastic fibers, implicating it in skin aging and photoaging processes. Related references: PMID:20876573
• The protein undergoes post-translational modifications including N-linked glycosylation, myristoylation, phosphorylation, and disulfide bond formation, which regulate its activity and stability.
• Mutations in the MME gene are associated with neurological disorders such as Charcot-Marie-Tooth disease and spinocerebellar ataxia, reflecting its functional importance beyond the immune system.
• Originally identified as the common acute lymphoblastic leukemia antigen (CALLA), CD10 is a widely used cell surface marker for hematopoietic cell immunophenotyping in flow cytometry.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 52–750, covering the majority of the extracellular domain. This design may favor recognition of the native surface-expressed CD10.
• For flow cytometry, consider including appropriate isotype controls and viability dyes to gate on live single cells.
• Adequate washing steps after staining are recommended to reduce background, especially when using directly conjugated fluorophores like .
• Note that CD10 can be shed from the cell surface; prolonged incubation or fixation before staining may affect detection.
• Validate antibody performance on known positive (e.g., B-lymphoblastoid cell lines) and negative (e.g., T-cell lines) controls in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated access to IVD raw materials and technical support, connecting diagnostic manufacturers and laboratories with high-quality antibodies, antigens, and ancillary reagents. This anti-CD10 monoclonal antibody serves as a reliable tool for hematopoietic malignancy immunophenotyping and other CD10-related research applications. Beyond this product, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk reagent sourcing to support your complete assay development pipeline.