Anti-CCT8 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P50990

Core Product Specifications and Parameters

Biological Background: CCT8 Function and Localization

• CCT8 is the theta subunit of the chaperonin-containing T-complex (TRiC), a multi-subunit molecular chaperone complex. Alternative names include CCT-theta, renal carcinoma antigen NY-REN-15, and chaperonin containing TCP1 subunit 8.
• The TRiC complex, including CCT8, assists in ATP-dependent folding of actin, tubulin, and other proteins, maintaining cytoskeletal integrity and proteostasis (PubMed:25467444, PubMed:36493755, PubMed:35449234). Related references: PMID:25467444 PMID:36493755 PMID:35449234
• CCT8/TRiC mediates the folding of WRAP53/TCAB1, a process critical for telomerase trafficking and telomere maintenance (PubMed:25467444). Related references: PMID:25467444
• The chaperonin complex is involved in the assembly of the BBSome, a ciliary trafficking complex, highlighting CCT8's role in ciliogenesis and vesicular transport to the cilium (PubMed:20080638). Related references: PMID:20080638
• CCT8 localizes predominantly to the cytoplasm, centrosomes, and the basal body of cilia, consistent with its role in cytoskeletal dynamics and ciliary function.
• Post-translational modifications of CCT8 include acetylation, phosphorylation, and ubiquitination (isopeptide bond formation), which may regulate its chaperone activity and interactions.

Experimental Guidance and Technical Tips

• For Western blotting (WB), the antibody detects a band at approximately 60 kDa corresponding to CCT8. Reduce nonspecific binding by optimizing blocking conditions and antibody dilution.
• In immunohistochemistry (IHC-P), ensure appropriate antigen retrieval and validate staining with positive and negative tissue controls.
• For ELISA, the polyclonal format may provide robust signal; however, consider pairing with a monoclonal capture antibody for enhanced specificity in quantitative assays.
• Given the high sequence conservation across human, mouse, and rat, this antibody is suitable for cross-species studies; nevertheless, confirm reactivity in your specific sample matrix.
• The immunogen (aa 248–497) lies within a region likely conserved across CCT8 orthologs; thus, the antibody may recognize other TRiC subunits? No, the immunogen is specific to CCT8; however, as a polyclonal, cross-reactivity with related subunits cannot be excluded—validate specificity in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with comprehensive raw-material supply and technical support. This anti-CCT8 antibody supports studies on protein folding, chaperonin biology, and ciliopathy research. Explore our portfolio of validated antibody pairs, recombinant proteins, and ancillary reagents to accelerate your assay development from concept to clinic.