Anti-CCT7 Polyclonal Antibody for WB, ELISA - Q99832

Core Product Specifications and Parameters

Biological Background: CCT7 Function and Localization

• CCT7 encodes T-complex protein 1 subunit eta, a component of the chaperonin-containing T-complex (TRiC) complex that assists protein folding.
• CCT7/TRiC mediates ATP-dependent folding of actin, tubulin, and other newly synthesized proteins.
  Related references:
  PMID:25467444 | PMID:36493755 | PMID:35449234
• The TRiC complex, with CCT7, folds WRAP53/TCAB1, regulating telomere maintenance.
  Related references:
  PMID:25467444
• CCT7 is also known as CCTH and NIP7-1; the protein interacts with HIV-1 Nef.
• Subcellular localization: CCT7 is predominantly cytoplasmic.
• Post-translational modifications of CCT7 include acetylation, methylation, and ubiquitin conjugation, which may modulate chaperonin activity.
• Alternative splicing generates multiple CCT7 isoforms, potentially expanding functional diversity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 310-500 of human CCT7, a region that may allow detection of multiple isoforms across species.
• For Western blot, consider using appropriate loading controls and validating antibody specificity in human, mouse, and rat lysates.
• For ELISA applications, optimize antigen coating concentration and detection conditions to achieve optimal signal-to-noise ratio.
• As CCT7 is a chaperonin, consider analyzing samples under conditions that alter protein folding, such as heat shock or proteasome inhibition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research laboratories with high-quality antibodies like this anti-CCT7 polyclonal antibody for protein folding and chaperonin research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, along with custom sourcing for rare targets. Explore our catalog to accelerate your assay development from concept to clinic.