Anti-CCT6B Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q92526

Core Product Specifications and Parameters

Biological Background: CCT6B (Probable T-complex protein 1 subunit zeta-2) Function and Localization

• CCT6B, also designated Cctz2, CCTZ-2, TSA303, CCT-zeta-2, or TCP-1-zeta-2, is a subunit of the eukaryotic chaperonin-containing T-complex (TRiC/CCT), a large barrel-shaped molecular chaperone complex.
• It functions in the ATP-dependent folding of newly synthesized proteins, predominantly actin and tubulin, thereby contributing to cytoskeletal assembly and cell cycle regulation.
• As a member of the TCP-1 chaperonin family, CCT6B shares structural homology with other TRiC subunits but is distinguished by its testis-specific expression pattern.
• Subcellular localization is predominantly cytoplasmic, where the TRiC complex facilitates co‑translational and post‑translational protein folding.
• Testis-specific expression suggests a specialized role in spermatogenesis, possibly in the folding of testis‑specific client proteins or during meiotic divisions.
• The protein is characterized by ATP‑binding and nucleotide‑binding domains, indicative of its chaperone‑assisted folding mechanism; metal‑binding and magnesium‑dependent hydrolase activity are also noted.
• Alternative splicing generates multiple transcript variants, which may modulate subunit composition and functional versatility within the TRiC complex.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 120–340 of human CCT6B, a region that may contain conserved epitopes; sequence alignment across species should be performed to predict cross‑reactivity with mouse and rat.
• For Western blotting, a band near 58 kDa is expected; use testis‑derived lysates or testicular cell lines as positive controls, given the testis‑specific expression of CCT6B.
• In immunofluorescence applications, fixation and permeabilization conditions should be optimized for cytoplasmic antigens; co‑localization with known cytoplasmic markers is advised to confirm specificity.
• When using ELISA, titrate both the coating antigen (recombinant protein or peptide) and primary antibody to achieve optimal signal‑to‑noise ratios.
• Consider validating antibody specificity using CCT6B knockout or knockdown samples in the appropriate cell type or tissue.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research laboratories with high‑quality reagents like this CCT6B polyclonal antibody. We offer access to validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents, with particular support for niche targets such as chaperonin complex components and testis‑specific proteins. From early‑stage development through clinical application, CamelBio streamlines your procurement process, ensuring reliable and timely delivery of critical materials.