Anti-CCT6A Rabbit Polyclonal Antibody for WB and ELISA - P40227

Core Product Specifications and Parameters

Biological Background: CCT6A Function and Localization

• CCT6A encodes the T-complex protein 1 subunit zeta, also known as TCP-1-zeta, CCT-zeta-1, or HTR3, a key subunit of the chaperonin-containing T-complex (TRiC).
• As a component of TRiC, CCT6A assists in the ATP-dependent folding of newly synthesized proteins, including actin and tubulin, ensuring proper cytoskeletal assembly. Related references: PMID:25467444, PMID:36493755, PMID:35449234
• TRiC also folds WRAP53/TCAB1, linking CCT6A to telomere maintenance and genomic stability. Related references: PMID:25467444
• CCT6A is part of a multi-gene family encoding eight distinct TRiC subunits (CCT1–CCT8).
• Subcellularly, CCT6A localizes predominantly to the cytoplasm, where it participates in chaperonin complexes.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, potentially modulating its chaperone activity.
• Alternative splicing yields multiple transcript variants, which may have tissue-specific functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved region (aa 80–250) of human CCT6A; cross-reactivity with rat CCT6A suggests epitope conservation. Validate specificity in your model system.
• For Western blot, expect a band near 58 kDa; using a reducing loading buffer may help resolve the protein.
• For ELISA, consider a direct or sandwich format with recombinant CCT6A protein as standard.
• As a polyclonal antibody, lot-to-lot variability can occur; include appropriate controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive selection of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents to support proteostasis and chaperone research. This anti-CCT6A antibody, along with other TRiC-related tools, helps streamline your diagnostic development from early-stage testing to clinical validation. Rely on CamelBio to source reliable, high-quality reagents for every project stage.