Anti-CCS Rabbit Polyclonal Antibody for WB and ELISA - O14618

Core Product Specifications and Parameters

Biological Background: CCS Function and Localization

• CCS is a copper chaperone that specifically delivers copper to copper-zinc superoxide dismutase (SOD1), an essential antioxidant enzyme.
• The protein is 274 amino acids in length with a molecular weight of approximately 29 kDa.
• CCS is ubiquitously expressed in human tissues, ensuring copper delivery to SOD1 across various cell types.
• It localizes primarily to the cytoplasm and cytosol.
• Post-translational modifications include disulfide bond formation, isopeptide bonds, phosphorylation, and ubiquitin-like conjugation, which may regulate its activity and stability.
• The protein contains metal-binding sites for copper, and possibly zinc, underscoring its role in metal ion homeostasis.

Experimental Guidance and Technical Tips

• The immunogen is a full-length recombinant CCS protein (amino acids 1–274), so the polyclonal antibody recognizes multiple epitopes.
• For Western blot, use a positive control such as HeLa or HEK293 cell lysate, which endogenously express CCS.
• Optimize primary antibody dilution and detection conditions in your specific sample system; start with a standard concentration range.
• Given the small size of CCS (29 kDa), use a 12% SDS-PAGE gel to achieve effective resolution.
• In ELISA, the antibody can be used for detection of recombinant or endogenous CCS, but validate coating and detection protocols.
• As a polyclonal antibody, expect some batch-to-batch variability; include appropriate controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For CCS-related research in oxidative stress and copper metabolism, we offer validated antibody pairs, optimized polyclonal antibodies like this CCS Rabbit pAb, bulk ancillary reagents, and rare target raw-material sourcing. Partner with us to streamline your assay development and biomarker discovery.