Anti-CCR9 Rabbit Monoclonal Antibody for WB, ELISA - P51686

Core Product Specifications and Parameters

Biological Background: CCR9 Function and Localization

• Receptor for the chemokine CCL25/TECK, triggering intracellular calcium mobilization and signal transduction.
• Functions as an alternative coreceptor with CD4 for HIV-1 infection, facilitating viral entry into host cells.
• Belongs to the G protein-coupled receptor family, characterized by seven-transmembrane domains.
• Localizes to the cell membrane and is highly expressed in the thymus, with lower levels in lymph nodes and spleen.
• Undergoes post-translational modifications including glycosylation and disulfide bond formation, typical of GPCRs.
• Alternatively spliced isoforms may exist; the canonical sequence yields a protein of approximately 42 kDa.
• Key structural features include a 3D-structure resolved, transmembrane helices, and conserved cysteine residues.
• Plays a role in lymphocyte trafficking and mucosal immunity, with potential involvement in inflammatory diseases.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to amino acids 1-100 of human CCR9 (NP_112477.1); epitope is likely within the N-terminal extracellular region.
• For Western blot, expect a specific band near 42 kDa; validate using thymus lysates where CCR9 is highly expressed.
• In ELISA, consider using the immunogen peptide as a positive control to confirm antibody binding.
• Cross-reactivity is reported for human, mouse, and rat; however, we recommend performing sequence alignment to verify epitope conservation across these species before use.
• For studies on HIV-1 co-receptor function, additional functional assays may be necessary to characterize the antibody's effect on viral entry.
• Due to the transmembrane nature of CCR9, using appropriate membrane protein extraction protocols is advisable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to high-quality IVD raw materials, including this anti-CCR9 monoclonal antibody. As your one-stop sourcing partner, we offer validated antibody pairs, bulk ancillary reagents, and custom rare-target raw-material procurement to streamline your chemokine receptor research and immunoassay development. Whether you need a specific clone, format, or conjugation, CamelBio bridges the gap from concept to clinic.