Anti-CCR7 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P32248

Core Product Specifications and Parameters

Biological Background: C-C chemokine receptor type 7 Function and Localization

• CCR7 (C-C chemokine receptor type 7) is a G protein-coupled receptor (GPCR) also known as BLR2, EBI1, CD197, and CDw197.
• It serves as the receptor for the chemokine MIP-3-beta (CCL19) and additionally binds CCL21, mediating lymphocyte homing and immune cell trafficking.
• CCR7 is a probable mediator of Epstein-Barr virus (EBV) effects on B-lymphocytes and contributes to normal lymphocyte functions, including migration to secondary lymphoid organs.
• The receptor localizes to the cell membrane and contains seven transmembrane domains characteristic of the GPCR superfamily.
• It is expressed in various lymphoid tissues and on activated B- and T-lymphocytes, with strong upregulation observed in EBV-infected B-cells and herpesvirus 6/7-infected T-cells.
• Post-translational modifications include glycosylation and disulfide bond formation; the protein has a calculated molecular weight of approximately 42.9 kDa (UniProt).
• Keywords from UniProt highlight its structural features: 3D-structure, disulfide bond, glycoprotein, and transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the intracellular C‑terminal region (aa 330–372); the antibody may recognize both unmodified and post‑translationally modified forms of CCR7.
• For western blotting, consider testing whole‑cell lysates from lymphoid tissues or activated lymphocyte lines; an expected band near 43 kDa should be verified.
• In immunofluorescence/ICC, permeabilization may be necessary for optimal detection of the intracellular epitope; membrane localization should be confirmed.
• Cross‑reactivity with mouse and rat has been reported; validate performance in the relevant species and experimental system.
• Polyclonal antibodies can exhibit lot‑to‑lot variability; it is advisable to include appropriate controls and consider consistency across batches.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibodies, antibody pairs, and ancillary reagents. Our CCR7‑related products support immunology, oncology, and infectious disease research, from biomarker discovery to assay development. Whether you require individual antibodies or complete reagent sets, CamelBio streamlines your supply chain to help accelerate your projects from concept to clinic.