Anti-CCR3 Rabbit Polyclonal Antibody (CKR3/CD193) for WB, IHC-P, ELISA - P51677

Core Product Specifications and Parameters

Biological Background: CCR3 Function and Localization

• G protein-coupled receptor (GPCR) regulating migration and activation of white blood cells in response to chemokines. Related references: PMID:28994588
• Selectively interacts with eosinophil-attracting chemokines such as eotaxin/CCL11, eotaxin-2/CCL24, and eotaxin-3/CCL26. Related references: PMID:7622448 PMID:8642344 PMID:8676064
• Signals through GNA14 or GNA16 to stimulate phospholipase C beta/PLCB2 and subsequent chemotaxis. Related references: PMID:18406577
• Alternatively transduces signals via GNAI1, leading to elevated intracellular calcium and activation of the PI3K/AKT pathway. Related references: PMID:8676064 PMID:35570218
• Acts as a possible functional receptor for NARS1. Related references: PMID:30171954
• Serves as an alternative coreceptor with CD4 for HIV-1 infection.
• Expressed primarily in eosinophils, with trace amounts in neutrophils and monocytes.
• Localizes to the cell membrane and contains conserved disulfide bonds characteristic of GPCRs.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region (aa 146–226) likely exposed on the extracellular side; polyclonal antibodies may recognize multiple epitopes.
• For Western blot, expect a major band around 41kDa; post-translational modifications (e.g., glycosylation) may cause additional bands or slight shifts.
• For IHC‑P, consider optimizing antigen retrieval and antibody dilution based on tissue fixation conditions.
• For ELISA, direct binding may be effective, but sandwich pair development requires further validation.
• Cross-reactivity with mouse and rat is reported, but verify in your specific sample system due to potential species differences.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable, one-stop access to IVD raw materials and technical support. This anti-CCR3 polyclonal antibody is ideal for immunology and infectious disease studies, particularly those focused on chemokine receptor biology. Beyond single antibodies, we supply validated antibody pairs, optimized recombinant monoclonal antibodies, and bulk ancillary reagents to streamline your assay development and production workflows.