Anti-CCR2 Rabbit Polyclonal Antibody for WB and ELISA - P41597

Core Product Specifications and Parameters

Biological Background: CCR2 Function and Localization

• CCR2 is a receptor for CCL2/MCP-1, CCL7, CCL12, and CCL13, mediating monocyte and macrophage chemotaxis via PI3K, Rac, and lamellipodium protrusion. It also functions as a receptor for DEFB106A/DEFB106B. Related references: PMID:23408426 PMID:38157855 PMID:8048929 PMID:23938203
• CCR2 is highly expressed on classical CD14+/CD16- monocytes and weakly on CD14+/CD16+ monocytes, type 2 DCs, and plasmacytoid DCs, with expression also detected on IL2-activated NK cells. Related references: PMID:9058802 PMID:38157855
• The protein is an integral membrane protein localized to the cell membrane, characterized by seven transmembrane domains typical of GPCRs.
• CCR2 undergoes several post-translational modifications, including disulfide bond formation, glycosylation, phosphorylation, and sulfation, which may regulate its function.
• It promotes Th17 differentiation during inflammation and facilitates thymocyte egress by enhancing S1P1R expression via FOXO1 signaling (by similarity).
• In the nervous system, CCR2 contributes to peripheral nerve injury-induced neuropathic pain and enhances NMDA receptor-mediated synaptic transmission via MAPK/ERK (by similarity). It also mediates macrophage recruitment to the brain after injury (by similarity).
• Alternative splicing generates isoforms such as CCR2A and CCR2B, and the protein is implicated in HIV-1 infection as a co-receptor with CD4.

Experimental Guidance and Technical Tips

• This polyclonal antibody, generated against a synthetic peptide spanning amino acids 150-250 of human CCR2, is designed for Western blot (WB) and ELISA applications. Its cross-reactivity with mouse suggests conserved epitopes, making it suitable for comparative studies.
• For WB, consider using whole-cell lysates from human or mouse monocyte/macrophage cell lines, and validate the observed band size (approximately 42 kDa) with appropriate positive and negative controls.
• In ELISA, pairing this antibody as a capture or detection reagent may be optimized by testing serial dilutions and confirming specificity with recombinant CCR2 protein.
• Given the transmembrane nature of CCR2, sample preparation with mild detergents could help preserve conformation for detection.
• Always titrate the antibody in your own experimental system to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner for IVD manufacturers and research institutes, providing a comprehensive portfolio of raw materials including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our CCR2 antibody supports research into chemokine signaling, inflammatory diseases, and neuroimmunology, and we specialize in sourcing rare-target raw materials to accelerate your diagnostic development from concept to clinic.