Anti-CCL4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P13236

Core Product Specifications and Parameters

Biological Background: CCL4 Function and Localization

• CCL4, also known as MIP-1β, ACT-2, LAG-1, and SCYA4, is a member of the CC chemokine family with a mature mass of ~10 kDa after signal peptide cleavage.
• It is a secreted monokine with inflammatory and chemokinetic properties, acting as a chemoattractant for various immune cells.
• CCL4 binds to the chemokine receptor CCR5, and is recognized as one of the major HIV-suppressive factors produced by CD8+ T-cells.
• Recombinant CCL4 dose-dependently inhibits infection by diverse strains of HIV-1, HIV-2, and SIV.
• The N-terminally processed form MIP-1β(3-69) retains the ability to down-modulate CCR5 surface expression and block CCR5-mediated HIV-1 entry, and also acts as a ligand for CCR1 and CCR2 isoform B.
• The protein contains conserved disulfide bonds critical for its chemokine structure and function, and is identified as a secreted cytokine involved in chemotaxis and inflammatory response.

Experimental Guidance and Technical Tips

• The immunogen was designed against a synthetic peptide covering the full-length mature human CCL4 sequence (aa 1-92), which may recognize both full-length and processed forms of CCL4.
• For Western blot (WB), the expected band is around 10 kDa; consider using low-molecular-weight markers and appropriate membrane type to retain small proteins, and validate in your specific sample lysate.
• For IF/ICC, as CCL4 is a secreted protein, optimize fixation and permeabilization conditions to capture extracellular or intracellular pools; include appropriate controls.
• For ELISA, this polyclonal antibody can potentially be used as a capture or detection reagent after proper pairing and validation in the relevant sample matrix.
• Cross-reactivity is indicated for human; if working with other species, verify sequence homology before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies like this anti-CCL4 pAb, and bulk ancillary reagents for immunoassay development. We also excel at sourcing rare target raw materials to support your research in chemokine biology, inflammation, and infectious diseases. Rely on CamelBio to efficiently bridge your supply chain from concept to clinic.