Anti-CCL1 (I-309) Rabbit Polyclonal Antibody for WB, ELISA - P22362

Core Product Specifications and Parameters

Biological Background: CCL1 Function and Localization

• CCL1, also known as I-309 or small-inducible cytokine A1, is a secreted C-C motif chemokine that functions as a chemoattractant for monocytes but not neutrophils.
• It exerts its biological effects through binding to the CCR8 receptor, playing a role in immune cell trafficking and inflammation.
• The protein is synthesized as a precursor with a signal peptide, which is cleaved to yield the mature secreted form.
• CCL1 contains highly conserved disulfide bonds characteristic of chemokines, contributing to its stable tertiary structure.
• Post-translational modifications include N-linked glycosylation, which may influence its activity and secretion.
• UniProt keywords highlight its roles in chemotaxis and cytokine activity, and it has been structurally characterized by 3D structure determination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature N-terminal region (amino acids 24-96), which is critical for receptor binding; thus, the resulting polyclonal antibody may recognize native and denatured forms.
• For Western blotting, a band around 11 kDa is expected under reducing conditions; consider using appropriate low-molecular-weight markers and PVDF membranes for optimal transfer.
• In ELISA, the antibody can be used for detection or capture; titrate concentrations and validate with recombinant CCL1 protein as standard.
• Since the antibody is raised in rabbit, use appropriate species-specific secondary antibodies and isotype controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, recombinant proteins, and ancillary reagents to accelerate your diagnostic development. This anti-CCL1 polyclonal antibody supports research into monocyte biology and inflammatory diseases, and we can assist with bulk orders, custom conjugation, and package deals for complete assay systems. From concept to clinic, CamelBio streamlines procurement so you can focus on your science.