Anti-CCKAR (CCK1-R) Rabbit Polyclonal Antibody for WB and ELISA - P32238

Core Product Specifications and Parameters

Biological Background: CCKAR (Cholecystokinin receptor type A) Function and Localization

• CCKAR, also known as the cholecystokinin-1 receptor (CCK1-R), is a G protein-coupled receptor encoded by the CCKAR gene in humans.
• It binds cholecystokinin with high affinity, exhibiting approximately 1000-fold greater affinity for CCK over gastrin.
• Upon activation, it mediates pancreatic growth and digestive enzyme secretion, as well as smooth muscle contraction of the gallbladder and stomach.
• In the central and peripheral nervous systems, CCKAR modulates feeding behavior and dopamine-induced behavioral responses.
• Signal transduction occurs via association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
• Localized primarily at the cell membrane, the receptor contains structural features such as transmembrane helices, disulfide bonds, and palmitoylation sites.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 100–200 of human CCKAR, which may favor recognition of the denatured or linear epitope.
• For Western blotting, consider using whole cell lysates from human tissues or cell lines known to express CCKAR (e.g., pancreas, gallbladder). A band near 48 kDa is expected, though glycosylation may cause slight shifts.
• For ELISA, the peptide immunogen can be used as a coating antigen, or the antibody may be applied in sandwich formats following pairing validation.
• Cross-reactivity with mouse CCKAR is reported, so the antibody can be tested in mouse models with appropriate controls.
• Optimal working dilutions for WB and ELISA must be determined empirically for each experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a leading one-stop IVD raw-material partner, providing diagnostic manufacturers and research labs with validated antibodies, bulk ancillary reagents, and full technical consulting from concept to clinic. This anti-CCKAR polyclonal antibody complements our portfolio of GPCR and gastrointestinal hormone receptor tools, and our sourcing team can help secure rare-target raw materials, optimized monoclonal/polyclonal pairs, and custom assay development support.