Anti-CCK Rabbit Polyclonal Antibody for WB and ELISA - P06307

Core Product Specifications and Parameters

Biological Background: Cholecystokinin Function and Localization

• Cholecystokinin (CCK) is a peptide hormone that plays a key role in gastrointestinal physiology by inducing gallbladder contraction and pancreatic enzyme secretion.
• CCK signals through two receptor subtypes: CCK-A receptors (primarily in the pancreas) stimulate amylase release, while CCK-B receptors (mainly in the stomach) mediate gastric acid secretion.
• The protein is synthesized as a precursor and undergoes proteolytic processing at paired basic residues to yield the biologically active hormone.
• CCK is a secreted protein found in the cerebrospinal fluid and urine, with its function in the brain still under investigation.
• Post-translational modifications include sulfation of tyrosine residues, C-terminal amidation, and glycosylation, which are critical for its biological activity.
• The predicted molecular weight of the mature peptide is approximately 12.7 kDa (UniProt), and the antibody detects a band around 13 kDa in experimental settings.
• CCK is part of the reference proteome and has a solved 3D structure, supporting its structural characterization.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 21–115 of the full-length CCK precursor, spanning the majority of the mature hormone; this design may enable detection of both precursor and processed forms.
• For Western blotting, note the low molecular weight (~13 kDa) and optimize gel concentration (e.g., 15% SDS-PAGE) and transfer conditions (e.g., low-molecular-weight cutoff membrane, 0.2 µm pore size) to retain the peptide.
• In ELISA applications, the polyclonal antibody can provide multi-epitope binding, potentially improving sensitivity; however, always include appropriate positive and negative controls.
• Given that CCK is a secreted peptide, sample preparation from tissues or cell lysates may require careful handling to prevent degradation; consider using protease inhibitors and chilled equipment.
• The reported cross-reactivity with mouse and rat suggests utility in rodent models, but species-specific validation in your own assays is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material supplier, offering researchers and manufacturers one-stop access to validated antibodies, bulk reagents, and technical resources. For CCK-related research in gastrointestinal and metabolic disorders, we provide polyclonal antibodies like this anti-CCK antibody, optimized for WB and ELISA. Beyond standard products, we specialize in sourcing rare targets and ancillary materials to support your entire diagnostic development pipeline, from concept to clinic.