Anti-CCDC98 (ABRA1) Rabbit Monoclonal Antibody for WB, IP, ELISA - Q6UWZ7

Core Product Specifications and Parameters

Biological Background: ABRAXAS1 (CCDC98) Function and Localization

• Protein identity: BRCA1-A complex subunit Abraxas 1, also known as coiled-coil domain-containing protein 98 (CCDC98) and FAM175A, encoded by the ABRAXAS1 gene.
• DNA damage response: Acts as a central scaffold protein in the BRCA1-A complex, which is essential for double-strand break (DSB) repair and genome stability maintenance.
• Recruitment mechanism: The BRCA1-A complex specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesion sites, facilitating the targeting of the BRCA1-BARD1 heterodimer to DSBs.
• Deubiquitinase activity: The complex exhibits deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, potentially modulating repair signaling.
• Subcellular localization: Predominantly localized to the nucleus, consistent with its role in DNA damage response.
• Post-translational modification: Classified as a phosphoprotein; phosphorylation may regulate its function and interaction dynamics within the BRCA1-A complex.
• Disease relevance: Disease variants of ABRAXAS1 have been linked to cancer, including predisposition to breast cancer, underscoring its clinical significance.
• Isoforms: Alternative splicing generates multiple isoforms, though detailed functional differences remain to be fully characterized.

Experimental Guidance and Technical Tips

• Immunogen design: Raised against a C-terminal peptide (amino acids 310–409). Confirm isoform specificity in your experimental model if alternative splicing products are a concern.
• Western blot (WB): Predicted molecular weight ~47 kDa. Include appropriate positive control cell lines (e.g., HeLa, HEK293) and consider validation via siRNA-mediated knockdown.
• Immunoprecipitation (IP): Suitable for IP; evaluate compatibility with your lysis buffer and optimize antibody concentration for efficient target capture.
• ELISA: Suitable for ELISA; for sandwich assays, pair with a non-overlapping detection antibody to ensure specificity.
• General: Monoclonal antibody offering high lot-to-lot consistency. Titrate to determine the optimal working dilution for your specific assay setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This CCDC98 (ABRAXAS1) antibody, targeting a pivotal DNA repair protein, is a valuable asset for oncology biomarker research and diagnostic assay development. Rely on CamelBio for validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and efficient sourcing of rare-target raw materials to accelerate your IVD pipeline.