Anti-CCDC92 Rabbit Polyclonal Antibody for WB, ELISA - Q53HC0

Core Product Specifications and Parameters

Biological Background: CCDC92 Function and Localization

• Coiled-coil domain-containing protein 92 (CCDC92), also known as Limkain beta-2, is an interferon-stimulated protein with a coiled-coil domain that suggests oligomerization capabilities.
• Functions in innate immunity by strongly inhibiting ebolavirus transcription and replication through direct interaction with viral ribonucleoprotein complexes.
• Acts by forming a complex with viral RNA-bound nucleocapsid protein (NP), thereby preventing the transport of NP to the cell surface and blocking viral assembly and release.
• Localizes to the cytoplasm, with enrichment at the centrosome and centriole within the microtubule organizing center, indicating a possible role in cytoskeletal regulation during antiviral responses.
• Subject to alternative splicing, producing isoforms that may modulate its antiviral activity or subcellular distribution.
• Classified as a phosphoprotein, suggesting that its activity or localization may be regulated by phosphorylation in response to immune signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-331) of human CCDC92. Consider that the antibody may detect isoforms containing this epitope region; validate specificity in your target cell lysates or tissue samples.
• For Western blotting, a band near 37 kDa is expected for the full-length protein. Use appropriate positive controls (e.g., interferon-stimulated cell lines) and confirm with siRNA knockdown or overexpression if possible.
• In ELISA applications, titrate the antibody to optimize signal-to-noise ratios for antigen capture or detection formats. Include recombinant CCDC92 protein as a standard for quantitative assays.
• This polyclonal antibody may exhibit batch-to-batch variability; it is advisable to characterize each new lot in pilot experiments before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For research on CCDC92 and antiviral innate immunity, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your assay development and biomarker discovery programs.