Anti-CCDC88A (Girdin) Polyclonal Antibody for WB, ELISA - Q3V6T2

Core Product Specifications and Parameters

Biological Background: CCDC88A/Girdin Function and Localization

• Protein identity and G protein modulation: CCDC88A encodes Girdin, a bifunctional G protein modulator that acts as a GEF for G(i) alpha subunits and a GDI for G(s) alpha, playing key roles in signal transduction (PubMed:19211784, PubMed:21954290, PubMed:23509302, PubMed:27621449). Related references: PMID:19211784, PMID:21954290, PMID:23509302, PMID:27621449
• Cell migration and EGFR signaling: Essential for cell migration; interacts with G(i) alpha subunits and EGFR to retain EGFR at the cell membrane, promoting downstream signaling and cell motility (PubMed:16139227, PubMed:19211784, PubMed:20462955). Related references: PMID:16139227, PMID:19211784, PMID:20462955
• PI3K-AKT pathway: Binding to G(i) alpha subunits enhances PI3K-dependent AKT1 phosphorylation, regulating GSK3 and FOXO1, and influencing DNA replication and cell proliferation (PubMed:19211784). Related references: PMID:19211784
• Actin cytoskeleton regulation: Required for formation of actin stress fibers and lamellipodia, ensuring cytoskeleton integrity (PubMed:15882442, PubMed:16139227, PubMed:19211784). Related references: PMID:15882442, PMID:16139227, PMID:19211784
• Ciliogenesis: Plays a role in cilium morphology and positioning, partly through regulation of CROCC/Rootletin localization (PubMed:27623382). Related references: PMID:27623382
• Subcellular localization: Localizes to the cell membrane, cytoplasm, lamellipodia, cilium basal body, and centrosome, reflecting its diverse roles in membrane trafficking and cytoskeletal organization.
• Tissue expression and modification: Ubiquitously expressed; alternative splicing generates multiple isoforms; subject to phosphorylation.
• Disease associations: Associated with epilepsy, intellectual disability, and neurodegeneration, consistent with its roles in neurogenesis and synaptic plasticity.

Experimental Guidance and Technical Tips

• For Western blot, use standard reducing conditions; a band of ~216 kDa is expected. Optimize primary antibody dilution starting at 1:500–1:1000, but validate in your sample system.
• For ELISA, consider coating at 1–10 µg/mL; include appropriate negative and positive controls.
• The immunogen corresponds to the C-terminal region (aa 1571–1870), which may allow detection of full-length protein and isoforms containing this region.
• Validate antibody specificity using knockdown or knockout experiments where possible.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio of validated antibodies, recombinant proteins, and ancillary reagents for diagnostic development. This anti-CCDC88A antibody is well-suited for studies in cell migration, cancer biology, and neurological disorders; our team can further assist with tailored sourcing of rare targets, matched antibody pairs, and bulk supply for your assay development needs.