Anti-CCDC61 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y6R9

Core Product Specifications and Parameters

Biological Background: CCDC61 Function and Localization

• CCDC61 is a centrosomal protein with coiled-coil domains, also called VFL3 homolog.
• It functions as a microtubule-binding protein required for centriole cohesion, independently of CEP250 and rootletin. Related references: PMID:31789463
• During interphase, CCDC61 anchors microtubules at the mother centriole subdistal appendages, contributing to centrosome positioning. Related references: PMID:31789463
• In mitosis, CCDC61 may regulate spindle assembly and chromatin alignment by organizing spindle microtubules symmetrically. Related references: PMID:30354798
• CCDC61 plays a non-essential role in ciliogenesis. Related references: PMID:31789463, PMID:32375023
• The protein localizes to centrosomes, centriolar satellites, and the cilium basal body.
• Post-translational modifications include phosphorylation and acetylation; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 352–531 of human CCDC61 (NP_001073871.1). For Western blotting, a band around 57 kDa is expected; however, actual migration may vary due to post-translational modifications or splicing variants.
• As a polyclonal antibody, it may detect multiple CCDC61 isoforms; validation in the relevant sample system is recommended.
• Suitable for ELISA; consider pairing with a capture antibody or using direct antigen coating, and include appropriate controls.
• Cross-reactivity has been validated with human; for other species, sequence homology should be checked before experimental use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material sourcing for diagnostic developers, offering a comprehensive portfolio that includes validated antibody pairs, optimized recombinant proteins, and bulk ancillary reagents. For centrosomal research targets like CCDC61, our team can assist with scaling up polyclonal antibodies or sourcing alternative clones to suit your assay development needs.