Anti-CCDC50 Rabbit Polyclonal Antibody for WB and ELISA - Q8IVM0

Core Product Specifications and Parameters

Biological Background: CCDC50 Function and Localization

• CCDC50 (Coiled-coil domain-containing protein 50), also known as YMER, is encoded by the CCDC50 gene located on chromosome 3. Multiple isoforms are produced through alternative splicing, with isoform 1 being the canonical form.
• The protein is involved in epidermal growth factor receptor (EGFR) signaling, likely functioning as a cytoplasmic adaptor or regulator in endocytic trafficking pathways downstream of EGFR activation.
• Subcellular localization studies show CCDC50 predominantly resides in the cytoplasm, consistent with its intracellular signaling role.
• Tissue-specific expression varies by isoform: isoform 1 and isoform 2 are coexpressed in placenta, liver, lung, kidney, and pancreas; only isoform 1 is detected in skeletal muscle, brain, and heart.
• Post-translational modifications such as acetylation and phosphorylation have been identified, suggesting regulatory mechanisms that may modulate CCDC50 activity and interactions.
• Mutations in CCDC50 are linked to non-syndromic deafness (DFNA44), indicating a critical role in auditory function, though the molecular mechanisms remain under investigation.
• As a proteomic reference component, CCDC50 serves as a model for studying coiled-coil domain proteins and their implications in disease.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 10-147 of human CCDC50, a region likely conserved across species, supporting reported cross-reactivity with human, mouse, and rat. Validate reactivity in your specific sample system using appropriate controls.
• In Western blot, expect a major band near 36 kDa (full-length isoform 1). Additional bands from alternative splicing isoforms or modifications may appear; confirm specificity by pre‑incubation with the immunogen peptide.
• As a polyclonal antibody, lot-to-lot variability is possible. Optimize dilution factors empirically for each new lot in your assay conditions.
• For ELISA, the antibody can be used as capture or detection reagent. Consider pairing with a matched monoclonal antibody to improve sensitivity and specificity, and validate with recombinant CCDC50 or known positive samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For research on cytoplasmic signaling proteins like CCDC50 involved in EGFR pathways, we provide one‑stop access to high‑quality reagents and technical support to accelerate your diagnostic development from concept to clinic.