Anti-CCDC47 Polyclonal Antibody for WB, IF/ICC, ELISA - Q96A33

Core Product Specifications and Parameters

Biological Background: CCDC47 Function and Localization

• CCDC47 is a subunit of the PAT complex, which is part of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer (PubMed:32814900, PubMed:32820719, PubMed:36261522). Related references: PMID:32814900, PMID:32820719, PMID:36261522
• Within the MPT complex, the PAT subcomplex sequesters highly polar regions in transmembrane domains away from the non-polar membrane environment until they can be buried in the fully assembled protein (By similarity).
• CCDC47 specifically occludes the lateral gate of the SEC61 complex, promoting the insertion of subsequent transmembrane regions (By similarity).
• In addition to its role in protein biogenesis, CCDC47 regulates calcium ion homeostasis in the endoplasmic reticulum, a function reflected by its alias "calumin" (PubMed:30401460). Related references: PMID:30401460
• It is required for proper protein degradation via the ER-associated degradation (ERAD) pathway, ensuring clearance of misfolded proteins (PubMed:25009997). Related references: PMID:25009997
• CCDC47 has an essential role in maintaining ER organization during embryogenesis (By similarity).
• The protein localizes to the endoplasmic reticulum membrane, specifically the rough ER, and features a coiled-coil domain, transmembrane helix, and glycosylation sites (UniProt Q96A33).

Experimental Guidance and Technical Tips

• The polyclonal antibody was generated using a recombinant fragment covering amino acids 224–483, which includes a portion of the coiled-coil domain; it is expected to recognize endogenous CCDC47 in samples from human, mouse, and rat.
• For western blotting (WB), a band around 56 kDa is predicted; always include appropriate positive and negative controls and validate specificity in your experimental system.
• In immunofluorescence (IF/ICC), fixation and permeabilization conditions should be optimized for your cell type; verify localization to the ER membrane.
• While ELISA reactivity is confirmed, note that polyclonal antibodies may exhibit lot-to-lot variation; consider performing a pilot titration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, from validated antibodies to ancillary reagents. For scientists investigating ER protein translocation, calcium signaling, or ERAD mechanisms, this anti-CCDC47 polyclonal antibody offers a reliable tool. Our one-stop sourcing capabilities include custom antibody development, bulk supply, and consulting services to streamline your journey from concept to clinic.