Anti-CCBL1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q16773

Core Product Specifications and Parameters

Biological Background: CCBL1 Function and Localization

• CCBL1 (KYAT1) is a pyridoxal phosphate-dependent aminotransferase that catalyzes the irreversible transamination of L-kynurenine to kynurenic acid, a broad-spectrum antagonist of ionotropic excitatory amino acid receptors. Related references: PMID:19338303, PMID:28097769
• It also functions as a cysteine-S-conjugate beta-lyase, metabolizing halogenated alkene/alkane cysteine conjugates to reactive metabolites. Related references: PMID:7883047
• The enzyme exhibits glutamine transaminase K (GTK) activity, converting glutamine and phenylpyruvate to alpha-ketoglutaramate and phenylalanine, respectively.
• Subcellular localization is predominantly cytosolic.
• The protein belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
• Alternative splicing produces multiple isoforms; the canonical isoform has a molecular weight of approximately 48 kDa (47875 Da from UniProt).
• Keywords include aminotransferase, lyase, pyridoxal phosphate, and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-270) of human CCBL1, which may allow detection of multiple isoforms where this region is conserved.
• For Western blotting, a specific band around 48 kDa (predicted molecular weight) is expected; however, actual migration may vary due to post-translational modifications or isoform differences.
• For immunofluorescence/ICC, appropriate fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) are recommended to access this cytosolic antigen.
• ELISA applications may require optimization of coating antigen and antibody concentration; consider using the full-length protein or immunogen fragment as a control.
• Validate antibody reactivity in the relevant sample systems (human, mouse, rat) using positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies high-quality CCBL1 antibodies and related IVD raw materials for tryptophan metabolism and cysteine conjugate beta-lyase research. As a one-stop sourcing partner, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom raw-material sourcing to support your assay development from concept to commercialization.