Anti-CCAR2 Rabbit Polyclonal Antibody for WB, IHC-P, and ELISA - Q8N163

Core Product Specifications and Parameters

Biological Background: CCAR2 Function and Localization

• CCAR2 (Cell Cycle and Apoptosis Regulator 2), also known as DBC-1, NET35, or KIAA1967, is a multi-domain nuclear and cytoplasmic protein implicated in transcriptional regulation, RNA processing, and apoptosis (UniProt Q8N163).
• As a core component of the DBIRD complex, CCAR2 integrates transcript elongation with alternative splicing, particularly affecting exons within (A+T)-rich DNA regions. Related references: PMID:22446626
• It inhibits SIRT1 deacetylase activity, thereby promoting p53/TP53 acetylation and p53-mediated apoptosis, establishing a critical link between metabolic sensing and tumor suppression. Related references: PMID:18235501 PMID:18235502 PMID:23352644
• CCAR2 represses SUV39H1 methyltransferase activity, influencing heterochromatin organization and gene silencing. Related references: PMID:19218236
• It mediates ligand-dependent transactivation by nuclear hormone receptors (e.g., ESR2, NR1H2, NR1H3) and modulates circadian clock components NR1D1 and BMAL1, thereby linking hormone signaling and circadian rhythms. Related references: PMID:19131338 PMID:20074560 PMID:23398316
• CCAR2 maintains genomic stability after UV stress and regulates gluconeogenesis via a mechanism involving NR1D1 and SIRT1. Related references: PMID:23398316 PMID:24415752
• It is ubiquitously expressed in normal tissues and frequently overexpressed in gastric, breast, lung, and colon carcinomas, consistent with its dual roles in cell cycle control and tumor suppression.
• Subcellular localization includes the nucleus, cytoplasm, and mitotic spindle, reflecting its dynamic involvement in chromatin regulation and cell division.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fusion protein spanning amino acids 654–923 of human CCAR2. This region lies in the C-terminal half, and the antibody may recognize multiple isoforms depending on your sample.
• For Western blot, a band at approximately 103 kDa is expected. Use appropriate loading controls and consider pre‑absorption with the immunogen peptide to confirm specificity.
• In IHC‑P, optimize antigen retrieval (e.g., citrate buffer pH 6.0 or Tris‑EDTA pH 9.0) and validate staining with known positive and negative tissues.
• For ELISA, pair with a suitable detection antibody and validate using recombinant CCAR2 protein to establish a standard curve.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw‑material procurement for diagnostic manufacturers and life science researchers. This anti‑CCAR2 antibody supports studies in apoptosis, transcriptional regulation, and cancer biology, and is backed by CamelBio’s one‑stop supply capability for validated antibody pairs, bulk ancillary reagents, and rare‑target sourcing, guiding projects from concept to clinic.