Anti-CCAR1 Polyclonal Antibody for WB, IP, ELISA - Q8IX12

Core Product Specifications and Parameters

Biological Background: CCAR1 Function and Localization

• CCAR1 (Cell division cycle and apoptosis regulator protein 1), also known as CARP-1 or DIS, is a multi-functional protein involved in transcriptional regulation, apoptosis, and cell cycle progression.
• It functions as a transcriptional coactivator and corepressor, associating with Mediator and p160 coactivator complexes to modulate nuclear receptor and p53-dependent transcription (By similarity).
• CCAR1 induces apoptosis in the presence of retinoid CD437 by sequestering 14-3-3 proteins and altering expression of cell cycle regulators such as MYC, CCNB1, and CDKN1A, thereby influencing cell proliferation. Related references: PMID:12816952
• During erythroid differentiation, CCAR1 cooperates with CALCOCO1 to enhance GATA1- and MED1-mediated transcriptional activation of the gamma-globin gene in K562 cells. Related references: PMID:24245781
• In prostate cancer cells, CCAR1 contributes to androgen receptor (AR)-mediated transcription by stabilizing AR-GATA2 association on chromatin and facilitating MED1 and RNA polymerase II recruitment, thereby promoting chromatin looping and tumorigenesis. Related references: PMID:23887938
• The protein localizes predominantly to the perinuclear cytoplasm, consistent with its role in signaling pathways.
• It is expressed in various epithelial cancer cell lines, including breast, colon, prostate, pancreatic, and leukemia, with expression regulated by growth factors.
• CCAR1 undergoes alternative splicing and post-translational modifications such as phosphorylation and ubiquitination, which may modulate its activity (UniProt keywords: Phosphoprotein, Ubl conjugation, Isopeptide bond).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-200), which may allow detection of multiple CCAR1 isoforms generated by alternative splicing. Confirm specificity in the relevant cell line.
• For Western blot (WB), CCAR1 has a predicted molecular weight of ~133 kDa. Use appropriate molecular weight markers and loading controls, and optimize blocking and antibody dilution.
• For immunoprecipitation (IP), consider using a standard protocol with protein A/G beads; validate the antibody's IP efficiency in your experimental system.
• For ELISA, use recombinant CCAR1 protein or cell lysates to optimize coating and detection conditions.
• Cross-reactivity with mouse and rat is expected based on sequence conservation; however, validate in the species and tissue of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. As a reliable sourcing partner, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target raw-material procurement. Our CCAR1 product supports research into apoptosis, cell cycle regulation, and cancer biology, helping you accelerate assay development and translational studies.