Anti-CBX8 Polyclonal Antibody for WB, IHC-P, ELISA - Q9HC52

Core Product Specifications and Parameters

Biological Background: CBX8 Function and Localization

• Chromobox protein homolog 8 (CBX8), also known as Polycomb 3 homolog (Pc3, hPc3) and Rectachrome 1, is a core component of the Polycomb group (PcG) multiprotein PRC1-like complex.
• CBX8 is essential for maintaining the transcriptionally repressive state of many genes, including Hox genes, throughout development via chromatin remodeling and histone modifications.
• The PRC1 complex containing CBX8 mediates monoubiquitination of histone H2A at 'Lys-119' (H2AK119ub1), thereby heritably altering chromatin expressibility and reinforcing gene silencing.
• CBX8 is predominantly localized in the nucleus, consistent with its role in chromatin regulation and transcriptional repression.
• As a member of the chromobox family, CBX8 functions as a chromatin regulator and transcriptional repressor, playing a key part in epigenetic memory and cell identity maintenance.
• The protein undergoes phosphorylation, as indicated by proteomics identification, potentially modulating its activity or interactions.
• The calculated molecular weight of CBX8 is approximately 43 kDa (UniProt: 43396 Da), aligning with the observed band in Western blot experiments.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 80-280 of human CBX8; consider this central region for mapping antibody binding and verifying sequence conservation across species.
• For Western blot, expect a specific band around 43 kDa; validate in positive control lysates from human, mouse, or rat cell lines expressing CBX8.
• In IHC-P, perform antigen retrieval and optimize primary antibody dilution; nuclear staining should be observed in tissue sections known to express CBX8.
• For ELISA, the antibody can be used as a capture or detection reagent after confirming specificity and sensitivity with recombinant CBX8 protein.
• Since this is a polyclonal antibody, note potential lot-to-lot variability and consider pre-validation in the intended sample type and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a one-stop sourcing solution for IVD raw materials, covering every stage from concept to clinic. This anti-CBX8 polyclonal antibody supports epigenetic and chromatin research, and CamelBio can further provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized raw-material sourcing to meet your project needs. We streamline your supply chain so you can focus on accelerating assay development and clinical translation.