Anti-CBX7 Polyclonal Antibody for WB - O95931

Core Product Specifications and Parameters

Biological Background: CBX7 Function and Localization

• Chromobox protein homolog 7 (CBX7) is a member of the chromobox family, characterized by a conserved chromodomain that binds methylated histones.
• Acts as a core component of the Polycomb repressive complex 1 (PRC1), which maintains transcriptional silencing of developmental genes such as Hox clusters.
• Catalyzes monoubiquitination of histone H2A at Lys119 (H2AK119ub), a hallmark of facultative heterochromatin and gene repression.
• Promotes trimethylation of histone H3 at Lys9 (H3K9me3), reinforcing a repressive chromatin environment.
• Directly binds trimethylated lysine residues on histone tails, linking histone modification readout to chromatin compaction.
• Functions as a regulator of cellular senescence by repressing the CDKN2A locus, thereby controlling cell cycle arrest independent of telomerase activity.
• Exclusively localizes to the nucleus, consistent with its role in chromatin regulation.
• Classified under keywords such as chromatin regulator, repressor, and transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 162–251) of human CBX7; consider that this region may be conserved across isoforms.
• This polyclonal antibody is recommended for Western blot (WB) detection of endogenous CBX7, with an expected band near 28 kDa.
• Optimal working dilution should be empirically determined; a starting range of 1:500–1:2000 is common for polyclonal sera in WB.
• Validate antibody performance in appropriate human cell lines (e.g., HeLa, HEK293) or tissue lysates known to express CBX7.
• Non-specific bands may appear due to polyclonal nature; include proper negative controls (e.g., knockout or siRNA-treated samples).

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CamelBio provides diagnostic manufacturers and laboratories with streamlined access to IVD raw materials, including validated antibodies like this anti-CBX7 polyclonal antibody for epigenetic and oncology research. Beyond this product, our sourcing network supports customized monoclonal antibody development, bulk ancillary reagents, and rare target material procurement to accelerate your assay development from concept to clinic.