Anti-CBX1/HP1 beta Monoclonal Antibody for WB, IHC-P, ELISA - P83916

Core Product Specifications and Parameters

Biological Background: CBX1/HP1 beta Function and Localization

• CBX1/HP1 beta, also known as heterochromatin protein 1 homolog beta (HP1β), p25beta, and modifier 1 protein (M31), is encoded by the CBX1 gene and belongs to the chromobox protein family.
• It is a key component of heterochromatin, where it specifically recognizes and binds histone H3 tails methylated at Lys-9 (H3K9me), thereby contributing to epigenetic repression. Related references: PMID:41094145
• CBX1/HP1 beta also recognizes and binds histone H1.4 methylated at Lys-26 (H1.4K26me). However, simultaneous phosphorylation at Ser-27 (H1.4S27Ph) excludes it from chromatin. Related references: PMID:16127177
• Interaction with lamin B receptor (LBR) can mediate the tethering of heterochromatin to the inner nuclear membrane (By similarity).
• Subcellular localization is predominantly nuclear, consistent with its role in chromatin organization.
• It is widely expressed in all adult and embryonic tissues.
• Post-translational modifications include phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation, highlighting its dynamic regulation.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a recombinant fusion protein covering amino acids 1-100 of human CBX1/HP1 beta. Consider verifying specificity by knockdown or overexpression in your experimental system.
• For Western blot (WB), a band at approximately 21 kDa is expected. Use appropriate positive controls (e.g., human, mouse, or rat tissue/cell lysates) and validate loading with housekeeping proteins.
• In immunohistochemistry (IHC-P), antigen retrieval may be necessary; optimize conditions for your specific tissue samples.
• For ELISA applications, titrate the antibody and include relevant standards; note that this antibody recognizes human, mouse, and rat CBX1.
• As a rabbit monoclonal antibody, it typically offers high specificity and lot-to-lot consistency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges the gap between IVD raw-material needs and reliable supply, offering a streamlined one-stop sourcing experience for diagnostic developers, research laboratories, and institutes. This anti-CBX1/HP1 beta monoclonal antibody serves as a robust tool for epigenetics and chromatin biology research, supporting applications such as western blotting, IHC, and ELISA. Through our comprehensive platform, clients can access validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets, ensuring a seamless transition from concept to clinical application.