Anti-CBX1 Polyclonal Antibody for WB, ELISA - P83916

Core Product Specifications and Parameters

| Parameter | Value | ||---|---| | Product Name | CBX1 Rabbit pAb | | Remarks/Alias | CBX; M31; MOD1; p25beta; HP1-BETA; HP1Hsbeta; HP1Hs-beta; CBX1 | | Species | Human | | GeneID (Human) | 10951 | | GeneID | 10951 | | Immunogen | Synthetic peptide|A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HP1 beta/CBX1 (NP_006798.1). | | Source | Rabbit | | Category | Polyclonal Antibodies | | Application | WB, ELISA | | Cross Reactivity | Human, Mouse, Rat | | SWISS | P83916 | | Protein Weight | 21kDa | | Shipping | Ice bag |

Biological Background: CBX1 Function and Localization

• CBX1 (Chromobox protein homolog 1, also known as HP1 beta) is a core component of heterochromatin that recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic transcriptional repression (PubMed:41094145).
  Related references:
  PMID:41094145
• CBX1 also binds histone H1.4 dimethylated at Lys-26 (H1.4K26me2); however, its chromatin occupancy is disrupted when H1.4 is simultaneously phosphorylated at Ser-27 (H1.4S27Ph) (PubMed:16127177).
  Related references:
  PMID:16127177
• Interaction with lamin B receptor (LBR) contributes to the association of heterochromatin with the inner nuclear membrane (By similarity).
• Subcellular localization is strictly nuclear.
• CBX1 is ubiquitously expressed in all adult and embryonic tissues.
• Post-translational modifications include phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation (according to UniProt keywords).
• The full-length human CBX1 protein has a calculated molecular weight of 21.4 kDa (UniProt: 21418 Da).

Experimental Guidance and Technical Tips

• For Western blot, expect a specific band around 21 kDa under standard denaturing and reducing conditions. Validate detection in your sample system, as endogenous CBX1 levels may vary.
• Optimize blocking and antibody dilution; polyclonal antibodies may show slight lot-to-lot variation. Use a suitable loading control (e.g., GAPDH or histone H3).
• For ELISA applications, consider coating with the N-terminal peptide (aa 1–100) or using a direct antigen capture format. The immunogen peptide can serve as a positive control.
• Because the antibody cross-reacts with mouse and rat CBX1, it is suitable for rodent model studies. Confirm reactivity in your specific species/tissue before quantitative assays.
• The epitope lies within the first 100 N-terminal amino acids, a region highly conserved across human, mouse, and rat.
• For chromatin-bound CBX1, ensure thorough nuclear extraction (e.g., RIPA buffer with sonication or nuclear extraction kit).
• To verify signal specificity, consider performing a peptide blocking assay with the immunogen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CBX1 polyclonal antibody supports your heterochromatin and epigenetic research needs. Beyond this product, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can assist with rare-target raw-material sourcing to accelerate your assay development.