Anti-CBS Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P35520

Core Product Specifications and Parameters

Biological Background: CBS Function and Localization

• CBS encodes cystathionine beta-synthase, a key enzyme in the transsulfuration pathway, converting homocysteine and serine to cystathionine, which is then converted to cysteine. Related references: PMID:20506325 PMID:23974653 PMID:23981774
• The enzyme is also implicated in the generation of hydrogen sulfide (H2S), a gasotransmitter with signaling and cytoprotective roles in neurons.
• Subcellularly, CBS localizes to the cytoplasm and nucleus.
• Tissue expression is highest in adult liver and pancreas, with lower levels in heart, brain, lung, and kidney; fetal expression is detected in brain, liver, and kidney.
• CBS is a heme- and pyridoxal phosphate-dependent allosteric enzyme, subject to regulation by S-adenosylmethionine.
• Post-translational modifications include phosphorylation and ubiquitination (isopeptide bond formation), influencing activity and stability.
• Genetic variants in CBS are associated with homocystinuria, a disorder of methionine metabolism.

Experimental Guidance and Technical Tips

• For Western blotting, the antibody detects a band at approximately 61 kDa; consider using reducing conditions and appropriate loading controls to confirm specificity.
• In immunofluorescence experiments, optimize fixation and permeabilization protocols for your cell type to ensure clear nuclear/cytoplasmic localization.
• For ELISA applications, the antibody can be employed as a capture or detection reagent; titrate coating concentrations to achieve optimal sensitivity.
• Given the cross-reactivity with mouse and rat, this antibody is suitable for comparative studies across species; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibodies for key metabolic targets like CBS, along with optimized antibody pairs, bulk ancillary reagents, and rare-target raw-material sourcing to support transsulfuration pathway research and assay development. Partner with CamelBio to streamline your sourcing and accelerate development of next-generation diagnostics.