Anti-CBR4 Rabbit Polyclonal Antibody for WB, ELISA - Q8N4T8

Core Product Specifications and Parameters

Biological Background: CBR4 Function and Localization

• CBR4, also known as SDR45C1, carbonyl reductase family member 4, or quinone reductase CBR4, is a 25 kDa short-chain dehydrogenase/reductase (SDR) enzyme.
• It is the beta-subunit of the heterotetrameric mitochondrial KAR complex, essential for mitochondrial fatty acid biosynthesis by reducing 3-oxoacyl-ACP to 3R-hydroxyacyl-ACP in an NADPH-dependent manner. Related references: PMID:25203508
• CBR4 homotetramers display NADPH-dependent quinone reductase activity towards a broad range of o- and p-quinones, suggesting a detoxification role against exogenous quinones. Related references: PMID:19000905 PMID:19571038
• The protein localizes to the mitochondrial matrix.
• CBR4 is detected at the protein level in liver and kidney, with highest expression in neuronal and muscle tissues. Related references: PMID:19000905 PMID:25203508
• Post-translational modifications include acetylation; alternative splicing produces isoforms, contributing to functional diversity.
• CBR4 belongs to the short-chain dehydrogenase/reductase superfamily and is classified under multiple gene ontology terms including fatty acid biosynthesis, lipid metabolism, and oxidoreductase activity, with key roles in mitochondrial pathways.

Experimental Guidance and Technical Tips

• The antibody was raised against full-length recombinant human CBR4 (aa 1-237), ensuring recognition of the native protein.
• For Western blotting, expect a specific band around 25 kDa; cross-reactivity with rat enables multi-species studies.
• In ELISA applications, consider using this antibody as a capture or detection reagent; validate binding conditions in your assay system.
• Upon receipt, store the antibody as recommended (e.g., at -20°C for long-term storage) after shipping on ice bags.
• As with any polyclonal antibody, we recommend titrating and validating performance in your specific sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents to drive diagnostic development. For research targeting mitochondrial fatty acid metabolism and quinone detoxification, this anti-CBR4 antibody is part of our broad portfolio, and we specialize in sourcing rare-target raw materials to support your project from concept to clinic. Reach out to explore custom options and one-stop supply solutions.