Anti-CBR1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P16152

Core Product Specifications and Parameters

Biological Background: CBR1 Function and Localization

• NADPH-dependent reductase with broad substrate specificity, reducing carbonyl compounds such as quinones, prostaglandins, menadione, and various xenobiotics.
• Converts antitumor anthracyclines doxorubicin and daunorubicin to cardiotoxic metabolites doxorubicinol and daunorubicinol. Related references: PMID:15799708, PMID:17344335, PMID:17912391
• Can convert prostaglandin E to prostaglandin F2-alpha (by similarity).
• Binds glutathione, showing higher affinity for glutathione-conjugated substrates; catalyzes reduction of S-nitrosoglutathione. Related references: PMID:17344335, PMID:18826943
• Participates in glucocorticoid metabolism by reducing cortisol/corticosterone to 20beta-dihydrocortisol/20beta-corticosterone, weak agonists of NR3C1 and NR3C2 in adipose tissue. Related references: PMID:28878267
• Subcellular localization: Cytoplasm
• Tissue specificity: Expressed in kidney at protein level.
• Keywords include Acetylation, Alternative splicing, Phosphoprotein, indicating post-translational modifications; also features 3D-structure, Direct protein sequencing, and Proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 100–200) of human CBR1. The antibody may recognize epitopes within this region across species, consistent with known cross-reactivity to mouse and rat.
• For Western blotting, a band around 30 kDa is expected. Validate with appropriate positive (e.g., kidney lysate) and negative controls.
• In IHC-P, optimize antigen retrieval and antibody dilution for the tissue of interest; note that CBR1 is expressed in kidney at the protein level.
• For ELISA, the recombinant immunogen can serve as a positive control; ensure optimal coating conditions and consider potential interference from reducing agents due to the enzyme's affinity for glutathione.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing for IVD manufacturers. Our expertise in redox metabolism and drug resistance research supports your development of diagnostic assays targeting carbonyl reductase 1 and related pathways. Partner with us to streamline your supply chain from concept to clinic.