Anti-CBR1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P16152

Core Product Specifications and Parameters

Biological Background: CBR1 Function and Localization

• Carbonyl reductase 1 (CBR1), also designated 15-hydroxyprostaglandin dehydrogenase, 20-beta-hydroxysteroid dehydrogenase, and PG-9-KR, is a member of the short-chain dehydrogenase/reductase family.
• Functions as an NADPH-dependent oxidoreductase with broad substrate specificity, reducing a variety of carbonyl compounds including quinones, prostaglandins, and xenobiotics.
• Mediates the reduction of antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic metabolites doxorubicinol and daunorubicinol. Related references: PMID:15799708 PMID:17344335 PMID:17912391
• Can bind glutathione, enhancing affinity for glutathione-conjugated substrates, and catalyzes the reduction of S-nitrosoglutathione. Related references: PMID:17344335 PMID:18826943
• Participates in glucocorticoid metabolism, reducing cortisol and corticosterone to 20beta-dihydrocortisol and 20beta-corticosterone, which are weak NR3C1/NR3C2 agonists in adipose tissue. Related references: PMID:28878267
• Localized in the cytoplasm and detected in kidney at the protein level.
• Subject to acetylation, alternative splicing, and phosphorylation; involved in lipid metabolism.

Experimental Guidance and Technical Tips

• The immunogen was designed against the C-terminal region (aa 178–277) of human CBR1, which may confer high specificity. Consider sequence alignment with homologous proteins if cross-reactivity is a concern.
• For Western blot, the predicted molecular weight is approximately 30 kDa. Validate detection in your sample system with appropriate positive and negative controls.
• In IHC-P, antigen retrieval conditions may require optimization. Pilot experiments with known positive tissues (e.g., kidney) are recommended.
• ELISA applications may benefit from testing a range of coating antigen concentrations and antibody dilutions to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including this Anti-CBR1 rabbit monoclonal antibody for applications in drug metabolism and glucocorticoid research. We provide validated antibody pairs, bulk ancillary reagents, and custom sourcing services to streamline your assay development from concept to clinic. With expertise spanning antibody discovery, optimization, and supply, CamelBio supports diagnostic manufacturers, labs, and research institutes in accelerating their projects with reliable, high-quality reagents.